Production of wheat doubled haploids by pollination with Job's tears (Coix lachryma-jobi. L.).
Wheat (Triticum aestivum L.) haploids were produced by crossing with Job's tears (Coix lachryma-jobi L.) as the pollen parent. Pollination was followed by 2,4-D treatment, detached tiller culture, and embryo culture, as described for maize pollination. The frequency of embryo formation was similar to that obtained by crossing wheat with maize pollen. Job's tears is a perennial plant which forms several stalks and its pollen can be collected throughout the year when the plant is maintained in a controlled environment. Our results indicate that Job's tears can be used as the pollen parent for wheat crosses for haploid production without requiring synchronization of flowering dates.[1]References
- Production of wheat doubled haploids by pollination with Job's tears (Coix lachryma-jobi. L.). Mochida, K., Tsujimoto, H. J. Hered. (2001) [Pubmed]
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