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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Isolation and characterization of a human thiamine pyrophosphokinase cDNA.

A human thiamine pyrophosphokinase cDNA clone (hTPK1) was isolated and sequenced. When the intact hTPK1 open reading frame was expressed as a histidine-tag fusion protein in Escherichia coli, marked enzyme activity was detected in the bacterial cells. The hTPK1 mRNA was widely expressed in various human tissues at a very low level, and the mRNA content in cultured fibroblasts was unaffected by the thiamine concentration of the medium. The chromosome localization of the hTPK1 gene was assigned to 7q34.[1]

References

  1. Isolation and characterization of a human thiamine pyrophosphokinase cDNA. Nosaka, K., Onozuka, M., Kakazu, N., Hibi, S., Nishimura, H., Nishino, H., Abe, T. Biochim. Biophys. Acta (2001) [Pubmed]
 
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