The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

A new indicator cell line to monitor human foamy virus infection and stability in vitro.

In order to determine the human foamy virus (HFV) infection in vitro conveniently, we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line (BHK-HFVLTR-EGFP) containing a plasmid that encodes the enhanced green fluorescent protein (EGFP) driven by the HFV long terminal repeat promoter. The viral trans-activator Bel-1 protein can induce EGFP expression, so the HFV titer could be determined by counting the corresponding EGFP-positive cells. This foamy-virus-activated EGFP expression ( FAE) assay was about 50 times more sensitive than the traditional focus plaque assay by end-point dilution. Additionally, the results of the FAE assay can be obtained rapidly within 2 days, whereas the determination of focus developing needs 2 weeks. Moreover, a linear relationship was found between the fluorescence intensity and the titer of inoculated HFV. In brief, the FAE assay is a rapid, easy, sensitive and quantitative method for monitoring and investigating HFV infection. Using the indicator cell line BHK-HFVLTR-EGFP, we examined the effects of repeated freeze-and-thaw cycles and temperature on HFV stability by the FAE assay. Such information about HFV infection and stability would be valuable for HFV applications.[1]


  1. A new indicator cell line to monitor human foamy virus infection and stability in vitro. Li, Z., Yang, P., Liu, H., Li, W.X. Intervirology (2002) [Pubmed]
WikiGenes - Universities