Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Ellena, J.F. et al.

Perfluorooctyl bromide has limited membrane solubility and is located at the bilayer center. Locating small molecules in lipid bilayers through paramagnetic enhancements of NMR relaxation.

There is considerable interest in the use of perfluorocarbons as oxygen carriers in clinical settings; however, little is known regarding the molecular interactions made by these apolar compounds with biological membranes or their effect on membrane structure. NMR spectroscopy was used to investigate the interaction of perfluorooctyl bromide (PFOB) with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers. (19)F NMR spectra demonstrate that PFOB partitions into POPC bilayers but that it saturates at a remarkably low membrane concentration of approximately 2 mol %. (19)F chemical shifts indicate that this membrane-bound PFOB experiences a local environment similar in polarity to that of hexane, suggesting that the compound resides within the hydrocarbon core of the lipid bilayer. This hydrocarbon location was refined by measuring paramagnetic enhancements of (19)F nuclear relaxation for membrane-bound PFOB produced by Gd(3+) and O(2). The data clearly localize PFOB to the center of the membrane hydrocarbon and show how paramagnetic enhancements of nuclear relaxation produced by O(2) may be used to localize small molecules within bilayers. (2)H and (31)P NMR experiments demonstrate that PFOB produces no significant changes in either acyl chain or headgroup structure even at saturating membrane concentrations.[1]

References

Perfluorooctyl bromide has limited membrane solubility and is located at the bilayer center. Locating small molecules in lipid bilayers through paramagnetic enhancements of NMR relaxation. Ellena, J.F., Obraztsov, V.V., Cumbea, V.L., Woods, C.M., Cafiso, D.S. J. Med. Chem. (2002) [Pubmed]

Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.