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Pezelj-Ribaric, S. et al.

Pezelj-Ribaric, Anic, Brekalo, Miletic, Hasan, Simunovic-Soskic,

Detection of tumor necrosis factor alpha in normal and inflamed human dental pulps.

BACKGROUND: The aim of this study was to determine concentrations of tumor necrosis factor-alpha (TNF-alpha) in normal, painful, and asymptomatic human dental pulps. METHODS: Pulps were obtained from three groups of teeth, including healthy teeth, asymptomatic teeth with caries and/or large restoration, and symptomatic teeth with clinical diagnosis of irreversible pulpitis. Pulpal tissues were collected, prepared, and analyzed for TNF-alpha concentration by ELISA technique. RESULTS: Nonparametric Kruskal-Wallis test revealed significant differences between TNF-alpha concentration in normal samples (64.01 +/- 53.12 pg/g) and irreversible symptomatic pulpal tissue (1962.99 +/- 1288.75 pg/g), between irreversible symptomatic and asymptomatic (1120.09 +/- 649.72 pg/g), and between normal and irreversible asymptomatic pulpal tissue (p = 0.000). CONCLUSIONS: TNF-alpha may be an objective marker for determining extent of pulpal inflammation associated with irreversible pulpitis.[1]

References

Detection of tumor necrosis factor alpha in normal and inflamed human dental pulps. Pezelj-Ribaric, S., Anic, I., Brekalo, I., Miletic, I., Hasan, M., Simunovic-Soskic, M. Arch. Med. Res. (2002) [Pubmed]

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