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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Rat liver homogenate hydrolases splitting N-acetyl-L-tyrosine ethyl ester.

Using gel filtration on Sephadex G 200, three fractions splitting N-acetyl-L-tyrosine ethyl ester (ATEE), termed I, S and II, were found in rat liver homogenate. In molecular size, electrophoretic mobility and thermolability, fractions I and II correspond to the ATEE-splitting enzymes contained in rat serum. The liver fraction S had a different surface electric charge and was relatively stable at high temperatures. Apart from ATEE, it splits certain ester and amide bonds of synthetic substrates. Purified fraction S increased vascular permeability in guinea pigs. Differential centrifugation of liver homogenate showed that fraction S and fractions I and II were localized differently in the subcellular particles.[1]

References

  1. Rat liver homogenate hydrolases splitting N-acetyl-L-tyrosine ethyl ester. Simonianova, E., Petáková, M., Jozová, O., Rybák, M. Physiologia Bohemoslovaca. (1975) [Pubmed]
 
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