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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Highly sensitive capillary zone electrophoresis with artificial seawater as the background electrolyte and transient isotachophoresis as the on-line concentration procedure for simultaneous determination of nitrite and nitrate in seawater.

Transient isotachophoresis-capillary zone electrophoresis with artificial seawater as the background electrolyte (BGE) was improved to further lower the limit of detection (LOD) for determination of nitrite and nitrate in seawater. By lowering the pH of BGE, the difference between effective mobility of nitrite and that of nitrate increased, thereby permitting increased sample volumes to be tolerated and their LOD values to decrease. Artificial seawater with pH adjusted to 3.0 using phosphate buffer was adopted as the BGE. To reverse electroosmotic flow (EOF), a capillary was flushed with 0.1 mM dilauryldimethylammonium bromide for 3 min before the capillary was filled with the BGE. Limits of detection (LODs) for nitrite and nitrate were 2.7 and 3.0 microg/l (as nitrogen), respectively. The LODs were obtained at a signal-to-noise ratio of 3. Values of the relative standard deviation (RSD) of peak area for these ions were 2.0 and 0.75%, respectively, when nitrite concentration was 0.05 mg/l and that of nitrate was 0.5 mg/l. The RSDs of peak height were 4.4 and 2.3%. The RSD values of migration time for these ions were 0.19 and 0.17%. The proposed method was applied to determination of nitrite and nitrate in a proposed certified reference material for nutrients in seawater, MOOS-1, distributed by the National Research Council of Canada. Results agreed with the assigned tolerance interval. This method was also applied to determination of these ions in seawater collected around Osaka Bay. Results nearly agreed with those obtained by a conventional spectrophotometric method.[1]


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