Imidocarb, a potent anti-protozoan drug, up-regulates interleukin-10 production by murine macrophages.
Interleukin-10 ( IL-10), a potent antiinflammatory and immunosuppressive cytokine, plays an important role in the regulation of immune responses. To discover small molecules that stimulate IL-10 production, a cell-based screening assay was performed using a murine macrophage cell line, RAW264. 7. Imidocarb, (3,3'-bis-2-imidazolin-2-yl)-carbanilide, which has been used as an anti-protozoan drug for the prevention and treatment of babesiosis in cattle, was thus identified. Imidocarb markedly enhanced LPS-induced IL-10 production not only by RAW264.7 cells but also by murine peritoneal macrophages in a concentration-dependent manner. It also augmented IL-10 production in the presence of zymosan, a yeast cell wall component. In contrast, imidocarb inhibited LPS-induced tumor necrosis factor (TNF)-alpha production by peritoneal macrophages. In mice, intraperitoneal administration of imidocarb significantly increased serum IL-10 levels and lowered TNF-alpha levels. Our results suggest that a novel anti-inflammatory property of imidocarb could lead to new therapeutic approaches in inflammatory conditions.[1]References
- Imidocarb, a potent anti-protozoan drug, up-regulates interleukin-10 production by murine macrophages. Katayama, T., Hayashi, Y., Nagahira, K., Konishi, K., Yamaichi, K., Oikawa, S. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
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