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Genetic transformation of Monascus purpureus DSM1379.

Monascus purpureus was transformed into hygromycin B resistance with hygromycin B phosphotransferase (hph) fused to Aspergillus nidulans trpC or a putative Monascus purpureus gpd1 promoter by electroporation. Among five strains, only M. purpureus DSM1397 was a competent recipient. Normal growth and sporulation on media containing up to 500 mg hygromycin B l(-1) occurred up to five generations. Upon transformation of the strain with the green fluorescent protein gene (sgfp) as a model gene and hph as a selection marker, characteristic green fluorescence was observed under fluoromicroscopy indicating successful transformation.[1]

References

  1. Genetic transformation of Monascus purpureus DSM1379. Kim, J.G., Choi, Y.D., Chang, Y.J., Kim, S.U. Biotechnol. Lett. (2003) [Pubmed]
 
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