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Disease relevance of Electroporation


High impact information on Electroporation

  • The human homolog of MAD2 was isolated and shown to be a necessary component of the mitotic checkpoint in HeLa cells by antibody electroporation experiments [6].
  • Derivatives of this vector were introduced into merozoites of P. berghei by electroporation, and parasites were selected for successful transformation in the rodent host on the basis of resistance to pyrimethamine [7].
  • To modulate Shh signaling in a spatiotemporally restricted manner, we carried out gain- and loss-of-function experiments in chick embryos using in ovo electroporation and found that Shh signaling is required for region-specific gene expression in thalamus and prethalamus, the major diencephalic brain areas flanking the ZLI [8].
  • Antigen binding and idiotype analysis of antibodies obtained after electroporation of heavy and light chain genes encoding phosphocholine-specific antibodies: a model for T15-idiotype dominance [9].
  • Expressing ephrin-A5 ectopically by in utero electroporation in the lateral cortex shifts the map of V1 medially, and expression within V1 disrupts its internal organization [10].

Chemical compound and disease context of Electroporation


Biological context of Electroporation


Anatomical context of Electroporation


Associations of Electroporation with chemical compounds

  • Stable hsp anti-sense IEC-18 cell clones were obtained by electroporation and hygromycin B selection [26].
  • Electroporation of monoclonal antibody (mAb) 1A4, which recognizes specifically the NH2-terminal Ac-EEED sequence of alpha-sm actin, significantly increased the frequency of migrating cells over that obtained with an unrelated antibody or a mAb against beta-actin [27].
  • Carrot protoplasts were used as a model system to optimize gene-transfer efficiency, which was measured 24-48 hr after electroporation by the amount of chloramphenicol acetyltransferase activity resulting from the expression of the introduced chimeric plasmids [28].
  • Hygromycin-resistant transformants of F. solani pisi generated by electroporation were assayed for CAT activity inducible by cutin hydrolysate and for glucose repression of this induction [29].
  • We have introduced three heterologous genes into T. vaginalis by electroporation and have used the 5' and 3' untranslated regions of the endogenous gene alpha-succinyl CoA synthetase B (alpha-SCSB) to drive transcription of these genes [30].

Gene context of Electroporation


Analytical, diagnostic and therapeutic context of Electroporation


  1. Possible role of calponin h1 as a tumor suppressor in human uterine leiomyosarcoma. Horiuchi, A., Nikaido, T., Taniguchi, S., Fujii, S. J. Natl. Cancer Inst. (1999) [Pubmed]
  2. Treatment of murine lupus with cDNA encoding IFN-gammaR/Fc. Lawson, B.R., Prud'homme, G.J., Chang, Y., Gardner, H.A., Kuan, J., Kono, D.H., Theofilopoulos, A.N. J. Clin. Invest. (2000) [Pubmed]
  3. Lysogeny and transformation in mycobacteria: stable expression of foreign genes. Snapper, S.B., Lugosi, L., Jekkel, A., Melton, R.E., Kieser, T., Bloom, B.R., Jacobs, W.R. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  4. Impairing actin filament or syndapin functions promotes accumulation of clathrin-coated vesicles at the apical plasma membrane of acinar epithelial cells. Da Costa, S.R., Sou, E., Xie, J., Yarber, F.A., Okamoto, C.T., Pidgeon, M., Kessels, M.M., Mircheff, A.K., Schechter, J.E., Qualmann, B., Hamm-Alvarez, S.F. Mol. Biol. Cell (2003) [Pubmed]
  5. Marked, transient inhibition of expression of the Epstein-Barr virus latent membrane protein gene in Burkitt's lymphoma cell lines by electroporation. Gahn, T.A., Sugden, B. J. Virol. (1993) [Pubmed]
  6. Identification of a human mitotic checkpoint gene: hsMAD2. Li, Y., Benezra, R. Science (1996) [Pubmed]
  7. Stable transfection of malaria parasite blood stages. van Dijk, M.R., Waters, A.P., Janse, C.J. Science (1995) [Pubmed]
  8. Hedgehog signaling from the ZLI regulates diencephalic regional identity. Kiecker, C., Lumsden, A. Nat. Neurosci. (2004) [Pubmed]
  9. Antigen binding and idiotype analysis of antibodies obtained after electroporation of heavy and light chain genes encoding phosphocholine-specific antibodies: a model for T15-idiotype dominance. Kenny, J.J., Moratz, C.M., Guelde, G., O'Connell, C.D., George, J., Dell, C., Penner, S.J., Weber, J.S., Berry, J., Claflin, J.L. J. Exp. Med. (1992) [Pubmed]
  10. Ephrin-as guide the formation of functional maps in the visual cortex. Cang, J., Kaneko, M., Yamada, J., Woods, G., Stryker, M.P., Feldheim, D.A. Neuron (2005) [Pubmed]
  11. Electrochemo-gene therapy of cancer: intratumoral delivery of interleukin-12 gene and bleomycin synergistically induced therapeutic immunity and suppressed subcutaneous and metastatic melanomas in mice. Kishida, T., Asada, H., Itokawa, Y., Yasutomi, K., Shin-Ya, M., Gojo, S., Cui, F.D., Ueda, Y., Yamagishi, H., Imanishi, J., Mazda, O. Mol. Ther. (2003) [Pubmed]
  12. Partial prevention of cisplatin-induced neuropathy by electroporation-mediated nonviral gene transfer. Pradat, P.F., Finiels, F., Kennel, P., Naimi, S., Orsini, C., Delaere, P., Revah, F., Mallet, J. Hum. Gene Ther. (2001) [Pubmed]
  13. Karyophilic properties of Semliki Forest virus nucleocapsid protein. Michel, M.R., Elgizoli, M., Dai, Y., Jakob, R., Koblet, H., Arrigo, A.P. J. Virol. (1990) [Pubmed]
  14. Apramycin resistance as a selective marker for gene transfer in mycobacteria. Paget, E., Davies, J. J. Bacteriol. (1996) [Pubmed]
  15. High efficiency introduction of plasmid DNA into glycine treated Enterococcus faecalis by electroporation. Cruz-Rodz, A.L., Gilmore, M.S. Mol. Gen. Genet. (1990) [Pubmed]
  16. Location of the cis-acting auxin-responsive region in the promoter of the par gene from tobacco mesophyll protoplasts. Takahashi, Y., Niwa, Y., Machida, Y., Nagata, T. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  17. Stable expression of selectable genes introduced into human hematopoietic stem cells by electric field-mediated DNA transfer. Toneguzzo, F., Keating, A. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  18. Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen loading of dendritic cells. Van Tendeloo, V.F., Ponsaerts, P., Lardon, F., Nijs, G., Lenjou, M., Van Broeckhoven, C., Van Bockstaele, D.R., Berneman, Z.N. Blood (2001) [Pubmed]
  19. Virus-mediated expression of firefly luciferase in the parasitic protozoan Giardia lamblia. Yu, D.C., Wang, A.L., Wu, C.H., Wang, C.C. Mol. Cell. Biol. (1995) [Pubmed]
  20. Acquisition of interleukin-3 independence in FDC-P2 cells after transfection with the activated c-H-ras gene using a bovine papillomavirus-based plasmid vector. Uemura, N., Ozawa, K., Tojo, A., Takahashi, K., Okano, A., Karasuyama, H., Tani, K., Asano, S. Blood (1992) [Pubmed]
  21. Evidence that embryonic neurons regulate the onset of cortical gliogenesis via cardiotrophin-1. Barnabé-Heider, F., Wasylnka, J.A., Fernandes, K.J., Porsche, C., Sendtner, M., Kaplan, D.R., Miller, F.D. Neuron (2005) [Pubmed]
  22. An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associates with the dividing nucleus. Traas, J.A., Doonan, J.H., Rawlins, D.J., Shaw, P.J., Watts, J., Lloyd, C.W. J. Cell Biol. (1987) [Pubmed]
  23. Localization and interaction of the cis-acting elements for abscisic acid, VIVIPAROUS1, and light activation of the C1 gene of maize. Kao, C.Y., Cocciolone, S.M., Vasil, I.K., McCarty, D.R. Plant Cell (1996) [Pubmed]
  24. Identification of a melanosomal membrane protein encoded by the pink-eyed dilution (type II oculocutaneous albinism) gene. Rosemblat, S., Durham-Pierre, D., Gardner, J.M., Nakatsu, Y., Brilliant, M.H., Orlow, S.J. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  25. Differential regulation of skeletal alpha-actin transcription in cardiac muscle by two fibroblast growth factors. Parker, T.G., Chow, K.L., Schwartz, R.J., Schneider, M.D. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  26. Interleukin-11-induced heat shock protein 25 confers intestinal epithelial-specific cytoprotection from oxidant stress. Ropeleski, M.J., Tang, J., Walsh-Reitz, M.M., Musch, M.W., Chang, E.B. Gastroenterology (2003) [Pubmed]
  27. A function for filamentous alpha-smooth muscle actin: retardation of motility in fibroblasts. Rønnov-Jessen, L., Petersen, O.W. J. Cell Biol. (1996) [Pubmed]
  28. Expression of genes transferred into monocot and dicot plant cells by electroporation. Fromm, M., Taylor, L.P., Walbot, V. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  29. Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor. Bajar, A., Podila, G.K., Kolattukudy, P.E. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  30. Transient and selectable transformation of the parasitic protist Trichomonas vaginalis. Delgadillo, M.G., Liston, D.R., Niazi, K., Johnson, P.J. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  31. Ectopic Myf5 or MyoD prevents the neuronal differentiation program in addition to inducing skeletal muscle differentiation, in the chick neural tube. Delfini, M.C., Duprez, D. Development (2004) [Pubmed]
  32. Nodal signaling induces the midline barrier by activating Nodal expression in the lateral plate. Yamamoto, M., Mine, N., Mochida, K., Sakai, Y., Saijoh, Y., Meno, C., Hamada, H. Development (2003) [Pubmed]
  33. FGF17b and FGF18 have different midbrain regulatory properties from FGF8b or activated FGF receptors. Liu, A., Li, J.Y., Bromleigh, C., Lao, Z., Niswander, L.A., Joyner, A.L. Development (2003) [Pubmed]
  34. Six3-mediated auto repression and eye development requires its interaction with members of the Groucho-related family of co-repressors. Zhu, C.C., Dyer, M.A., Uchikawa, M., Kondoh, H., Lagutin, O.V., Oliver, G. Development (2002) [Pubmed]
  35. Msx1 is required for dorsal diencephalon patterning. Bach, A., Lallemand, Y., Nicola, M.A., Ramos, C., Mathis, L., Maufras, M., Robert, B. Development (2003) [Pubmed]
  36. Localization of a mammalian homolog of diaphanous, mDia1, to the mitotic spindle in HeLa cells. Kato, T., Watanabe, N., Morishima, Y., Fujita, A., Ishizaki, T., Narumiya, S. J. Cell. Sci. (2001) [Pubmed]
  37. Poly-L-glutamate, an anionic polymer, enhances transgene expression for plasmids delivered by intramuscular injection with in vivo electroporation. Nicol, F., Wong, M., MacLaughlin, F.C., Perrard, J., Wilson, E., Nordstrom, J.L., Smith, L.C. Gene Ther. (2002) [Pubmed]
  38. Electroporation-mediated tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)/Apo2L gene therapy for hepatocellular carcinoma. Yamashita, Y., Shimada, M., Tanaka, S., Okamamoto, M., Miyazaki, J., Sugimachi, K. Hum. Gene Ther. (2002) [Pubmed]
  39. Pak1 is involved in dendrite initiation as a downstream effector of Rac1 in cortical neurons. Hayashi, K., Ohshima, T., Mikoshiba, K. Mol. Cell. Neurosci. (2002) [Pubmed]
  40. In vivo electroporation of proopiomelanocortin induces analgesia in a formalin-injection pain model in rats. Lee, T.H., Yang, L.C., Chou, A.K., Wu, P.C., Lin, C.R., Wang, C.H., Chen, J.T., Tang, C.S. Pain (2003) [Pubmed]
  41. Estimation of methods of protein delivery into mammalian cells--a comparative study by electroporation and bioporter assay. Todorova, R. Prikl. Biokhim. Mikrobiol. (2009) [Pubmed]
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