Haemolytic activities and immunologic adjuvant effect of Panax notoginseng saponins.
AIM: To evaluate haemolytic activities and adjuvant effect of Panax notoginseng saponins (PNS). METHODS: The haemolytic potential of PNS and its effects on the humoral and cellular immune responses to mice subcutaneously immunized with ovalbumin ( OVA) were examined. ICR mice were immunized with OVA 100 microg alone, the mixture of OVA 100 microg and aluminoid 2 mg, or mixture of OVA 100 microg and PNS 50, 100, or 200 microg on the first and fifteenth day. Two weeks later (d 28), antigen-specific antibody in serum and concanavalin A ( Con A)-, pokeweed (PWM)-, and phytohaemagglutinin (PHA)-stimulated splenocyte proliferation were investigated. RESULTS: Haemolytic percents of PNS-treated red blood cell were 11.6 % and 3.6 % at concentrations of 500 and 250 mg/L, respectively. Con A-, PWM- and PHA-stimulated splenocyte proliferation in the mice immunized with OVA/PNS (100 microg) was higher than that in the OVA control group (P<0.01). Mice immunized with OVA and PNS (50, 100, 200 microg) showed significantly higher serum OVA-specific IgG antibody titers (P<0.01) in comparison with the control group that received OVA alone. CONCLUSION: PNS possesses immunologic adjuvant activities and low-haemolytic effect.[1]References
- Haemolytic activities and immunologic adjuvant effect of Panax notoginseng saponins. Sun, H.X., Pan, H.J., Pan, Y.J. Acta Pharmacol. Sin. (2003) [Pubmed]
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