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Detection of Cryptosporidium parvum in soil extracts.

Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precision. Background debris may have produced overestimates at low oocyst concentrations when flow cytometry was used.[1]

References

  1. Detection of Cryptosporidium parvum in soil extracts. Walker, M., Redelman, D. Appl. Environ. Microbiol. (2004) [Pubmed]
 
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