A Tobacco necrosis virus D isolate from Olea europaea L.: viral characterization and coat protein sequence analysis.
A virus isolated from Olea europaea L. grown in Portugal, was identified as a member of the species Tobacco necrosis virus D (TNV-D, genus Necrovirus, family Tombusviridae), based on the molecular and serological properties of the purified virus particles. The genomic region encoding the coat protein ( CP) of this isolate (named GP isolate) was amplified by RT-PCR and the cDNA was cloned and sequenced. The CP gene encodes a predicted protein of 269 amino acids showing high identity (86.2%) to TNV-D coat protein sequence. Phylogenetic analysis based on necroviruses CP sequences, confirmed GP as a TNV-D isolate. The alignment with homologous TNV-D CP sequences revealed four conserved amino acids involved in Ca(2+) binding as well as the plant virus icosahedral capsid protein "S' signature. Based on the determined nucleotide sequence, specific primers were designed and successfully used in RT-PCR for virus diagnosis in naturally infected olive trees.[1]References
- A Tobacco necrosis virus D isolate from Olea europaea L.: viral characterization and coat protein sequence analysis. Cardoso, J.M., Félix, M.R., Oliveira, S., Clara, M.I. Arch. Virol. (2004) [Pubmed]
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