Disease relevance of Plant Viruses

• The identification of this symptom-modulating RNA as a co-linear deletion mutant of the helper virus genome establishes it as the first definitive defective interfering RNA (DI RNA) to be identified in association with a plant virus [1].
• Many of the residues that the TMV read-through domain and the segmented plant viruses have in common are also conserved in a read-through domain found in the nonstructural polyprotein of the animal alphaviruses Sindbis and Middelburg [2].
• The three-dimensional structure of an siRNA bound to the tombusvirus p19 protein--a suppressor of gene silencing--provides a first glimpse into how plant viruses can defeat their host's anti-viral RNAi defenses [3].
• METHODS: An infectious plant virus clone, p35SZYMV2-26, that contains the full-length cDNA to the genomic RNA of a Taiwan isolate of ZYMV, driven by the cauliflower mosaic virus 35S promoter, was engineered as an in vivo viral vector to express Dermatophagoides pteronyssinus group 5 allergen (Der p 5) in cucurbit species [4].
• The 3a movement protein (MP) of a plant virus, Cucumber mosaic virus (CMV), forms ribonucleoprotein (RNP) complexes with viral RNA, capable of trafficking from cell-to-cell throughout the infected plant only in the presence of the CMV capsid protein (CP) [5].

High impact information on Plant Viruses

• Biochemical and mutational analysis of a plant virus polyprotein cleavage site [6].
• Transgenic plants expressing PAP are resistant to a broad spectrum of plant viruses [7].
• Induction of HSP70 and polyubiquitin expression associated with plant virus replication [8].
• To test the possibility of engineering a broad type of resistance to plant viruses, we expressed the antisense RNA for tobacco SAHH in transgenic tobacco plants [9].
• The natural role of PTGS seems to be as a defence against plant viruses, so what first appeared to be RNAs on the attack may now be considered RNAs on the defense [10].

Chemical compound and disease context of Plant Viruses

• Functional replacement of the tobacco rattle virus cysteine-rich protein by pathogenicity proteins from unrelated plant viruses [11].
• SAR1 is a new IgG2a murine monoclonal antibody derived by immunization with a plant virus expressing the sequence GERDRDR from the C-terminal tail of the gp41 transmembrane glycoprotein of human immunodeficiency virus type 1 (HIV-1) [12].
• The M dsRNA-encoded putative replicase protein contained the conserved methyl transferase, helicase and RNA-dependent RNA polymerase (RdRp) domains characteristic of the replicases of potex-like plant viruses (flexiviruses) and Botrytis virus F (BVF), a flexuous rod mycovirus infecting the phytopathogenic fungus Botrytis cinerea [13].
• The behavior of salt-labile plant viruses in gradients of cesium sulphate [14].
• Stimulation and inhibition of plant virus replication in vivo by 6-benzylaminopurine [15].

Biological context of Plant Viruses

• Molecular genetic analysis of a plant virus polyprotein cleavage site: a model [16].
• Phylogeny of capsid proteins of small icosahedral RNA plant viruses [17].
• By analogy to other tripartite plant viruses, it is presumed that the first ORF (867 nucleotides) codes for the 3a protein and the second ORF (654 nucleotides) is the cistron of the coat protein which is expressed from subgenomic RNA 4 of 1010 nt residues [18].

Gene context of Plant Viruses

• Identification of the function of the 30K protein was especially important because it was the first evidence that a plant virus possesses a cell-to-cell movement function [19].
• The PhMV RP also possesses the methyl transferase, polymerase and the helicase motifs found in all the Sindbis-like super group of plant viruses [20].
• Structural characterization of HC-Pro, a plant virus multifunctional protein [21].
• This is the first report that a region of MP determines the requirement of CP in cell-to-cell movement of a plant virus [22].
• A viable mutation in cauliflower mosaic virus, a retroviruslike plant virus, separates its capsid protein and polymerase genes [23].

Analytical, diagnostic and therapeutic context of Plant Viruses

• We developed an in situ hybridization technique for localization of plant virus RNA in ultra-thin sections of virus-infected, Lowicryl HM20- or osmium-fixed, Araldite-embedded plant leaf tissues [24].
• Isopycnic centrifugation of plant viruses in Nycodenz density gradients [25].
• We tested this repeat ELISA (R-ELISA) procedure on high antigen-binding ELISA plates coated with two different plant virus proteins, a synthetic peptide, the p25/24 gag and the gp120 proteins of the human immuno-deficiency virus, or the staphylococcus enterotoxin protein [26].

References