Disease relevance of Tombusviridae

• Mutant movement proteins of red clover necrotic mosaic dianthovirus (RCNMV), consisting of in-frame deletions or fusions with a maltose-binding protein, were produced in Escherichia coli using expression vectors [1].
• The tentative phylogenetic tree derived from the capsid protein alignment separated into three main lineages: (I) carmo-, tombus- and dianthoviruses, (II) southern bean mosaic, tobacco necrosis and maize chlorotic mottle viruses, and (III) luteoviruses [2].
• These primers were shown to be efficient in PCR screening of different RdRp genes including those of carmoviruses, dianthoviruses, and tombusviruses [3].

High impact information on Tombusviridae

• Aureusvirus P14 is an efficient RNA silencing suppressor that binds double-stranded RNAs without size specificity [4].
• The furovirus 37K proteins were found to reside in a clade that also contained the dianthovirus MPs [5].
• Isolation and characterization of an RNA-dependent RNA polymerase from Nicotiana clevelandii plants infected with red clover necrotic mosaic dianthovirus [6].
• The putative RNA-dependent RNA polymerase shows 91.2% amino acid identity with that of an isolate of Olive latent virus 1 (OLV-1) and the coat protein reveals highest sequence identity with that of TNV-D [7].
• Phylogenetic analysis based on necroviruses CP sequences, confirmed GP as a TNV-D isolate [8].

Gene context of Tombusviridae

• The 37-kDa protein has sequence similarity with the cell-to-cell transport protein of dianthoviruses [9].

References