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Cloning and mapping of the CYS4 gene of Saccharomyces cerevisiae.

A DNA fragment containing the CYS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2 microns DNA chromosome-loss procedure, the cys2 and cys4 mutations, which are linked together and co-operatively confer cysteine dependence, were assigned to chromosome VII. By further mapping involving tetrad analysis, the cys2-cys4 pair was localized between SUP77 (SUP166) and ade3 on the right arm of chromosome VII.[1]

References

  1. Cloning and mapping of the CYS4 gene of Saccharomyces cerevisiae. Ono, B., Heike, C., Yano, Y., Inoue, T., Naito, K., Nakagami, S., Yamane, A. Curr. Genet. (1992) [Pubmed]
 
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