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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Crystalline enzyme kinetics: activity of the Streptomyces R61 D-alanyl-D-alanine peptidase.

The specificity constant, kcat/Km, for the hydrolysis of hippuryl-mercaptoacetate by crystals of the Streptomyces R61 D-D peptidase was measured by reaction of the thiol produced with 4,4'-dithiodipyridine. The values of kcat/Km for the crystal and in solution were the same (within experimental error). A novel method for treating the lag in the progress curves was developed.[1]

References

  1. Crystalline enzyme kinetics: activity of the Streptomyces R61 D-alanyl-D-alanine peptidase. Kelly, J.A., Waley, S.G., Adam, M., Frère, J.M. Biochim. Biophys. Acta (1992) [Pubmed]
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