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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody.

High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.[1]


  1. Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody. Chan, P.K., To, W.K., Liu, E.Y., Ng, T.K., Tam, J.S., Sung, J.J., Lacroix, J.M., Houde, M. J. Med. Virol. (2004) [Pubmed]
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