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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene.

Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing rapeseed embryos during storage lipid biosynthesis. The resulting transgenic plants showed dramatically increased stearate levels in the seeds. A continuous distribution of stearate levels from 2% to 40% was observed in seeds of a transgenic B. napus plant, illustrating the potential to engineer specialized seed oil compositions.[1]

References

  1. Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Knutzon, D.S., Thompson, G.A., Radke, S.E., Johnson, W.B., Knauf, V.C., Kridl, J.C. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
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