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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Gene Transfer Techniques

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Disease relevance of Gene Transfer Techniques


Psychiatry related information on Gene Transfer Techniques


High impact information on Gene Transfer Techniques

  • Using lentiviral transgenesis, we generated NOD mice in which Slc11a1 is silenced by RNAi [7].
  • There is compelling evidence from mutation analysis and transgenesis that the SRY gene isolated from human and mouse encodes the testis-determining factor on the mammalian Y chromosome [8].
  • Here we show, using gene-transfer techniques, that beclin 1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells [9].
  • When glut2 was expressed by transgenesis in glial cells but not in neurons of ripglut1;glut2-/- mice, stimulated glucagon secretion was restored as was c-Fos-like immunoreactive labeling in the brainstem [10].
  • Yeast artificial chromosome-based transgenesis was used to overexpress a disease-associated mutant form of human fibrillin-1 (C1663R) on a normal mouse background [11].

Biological context of Gene Transfer Techniques


Anatomical context of Gene Transfer Techniques


Associations of Gene Transfer Techniques with chemical compounds


Gene context of Gene Transfer Techniques

  • We next combined phylogenetic footprinting, transient transgenesis, and confocal imaging methods to study the Ci-pitx cis-regulatory system, with special emphasis on the regulation of isoform-specific ANB/stomodaeal expression [27].
  • Functional rescue of the glomerulosclerosis phenotype in Mpv17 mice by transgenesis with the human Mpv17 homologue [28].
  • Human and mouse ABCA1 comparative sequencing and transgenesis studies revealing novel regulatory sequences [29].
  • We have used BAC transgenesis in the mouse to begin to delineate the region of DNA required for proper expression and imprinting of the mouse Delta-like1 (Dlk1) and Gene-trap locus2 (Gtl2) imprinted genes [30].
  • The authors also discuss the further testing of one candidate, the phospholipase gene Pla2g2a, by transgenesis [31].

Analytical, diagnostic and therapeutic context of Gene Transfer Techniques


  1. Mouse homologues of human hereditary disease. Searle, A.G., Edwards, J.H., Hall, J.G. J. Med. Genet. (1994) [Pubmed]
  2. Expression of the cAMP response element binding protein (CREB) in hippocampus produces an antidepressant effect. Chen, A.C., Shirayama, Y., Shin, K.H., Neve, R.L., Duman, R.S. Biol. Psychiatry (2001) [Pubmed]
  3. Overexpression of urokinase receptor in breast cancer cells results in increased tumor invasion, growth and metastasis. Xing, R.H., Rabbani, S.A. Int. J. Cancer (1996) [Pubmed]
  4. The promoter of the long terminal repeat of feline leukemia virus is effective for expression of a mouse H-2 histocompatibility gene in mouse and human cells. Wong, T.C., Goodenow, R.S., Sher, B.T., Davidson, N. Gene (1985) [Pubmed]
  5. Synaptotagmin I hypothalamic knockdown prevents amygdaloid seizure-induced damage of hippocampal neurons but not of entorhinal neurons. Kobayashi, S., Ohno, K., Iwakuma, M., Kaneda, Y., Saji, M. Neurosci. Res. (2002) [Pubmed]
  6. Involvement of glial glutamate transporters in morphine dependence. Nakagawa, T., Satoh, M. Ann. N. Y. Acad. Sci. (2004) [Pubmed]
  7. In vivo RNA interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Kissler, S., Stern, P., Takahashi, K., Hunter, K., Peterson, L.B., Wicker, L.S. Nat. Genet. (2006) [Pubmed]
  8. Widespread expression of the testis-determining gene SRY in a marsupial. Harry, J.L., Koopman, P., Brennan, F.E., Graves, J.A., Renfree, M.B. Nat. Genet. (1995) [Pubmed]
  9. Induction of autophagy and inhibition of tumorigenesis by beclin 1. Liang, X.H., Jackson, S., Seaman, M., Brown, K., Kempkes, B., Hibshoosh, H., Levine, B. Nature (1999) [Pubmed]
  10. Regulation of glucagon secretion by glucose transporter type 2 (glut2) and astrocyte-dependent glucose sensors. Marty, N., Dallaporta, M., Foretz, M., Emery, M., Tarussio, D., Bady, I., Binnert, C., Beermann, F., Thorens, B. J. Clin. Invest. (2005) [Pubmed]
  11. Evidence for a critical contribution of haploinsufficiency in the complex pathogenesis of Marfan syndrome. Judge, D.P., Biery, N.J., Keene, D.R., Geubtner, J., Myers, L., Huso, D.L., Sakai, L.Y., Dietz, H.C. J. Clin. Invest. (2004) [Pubmed]
  12. The oncogenic T cell LIM-protein Lmo2 forms part of a DNA-binding complex specifically in immature T cells. Grütz, G.G., Bucher, K., Lavenir, I., Larson, T., Larson, R., Rabbitts, T.H. EMBO J. (1998) [Pubmed]
  13. The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish. Ward, A.C., McPhee, D.O., Condron, M.M., Varma, S., Cody, S.H., Onnebo, S.M., Paw, B.H., Zon, L.I., Lieschke, G.J. Blood (2003) [Pubmed]
  14. A BAC transgenic analysis of the Mrf4/Myf5 locus reveals interdigitated elements that control activation and maintenance of gene expression during muscle development. Carvajal, J.J., Cox, D., Summerbell, D., Rigby, P.W. Development (2001) [Pubmed]
  15. Interaction of alpha 1 with alpha 2 region in class I MHC proteins contributes determinants recognized by antibodies and cytotoxic T cells. Stroynowski, I., Clark, S., Henderson, L.A., Hood, L., McMillan, M., Forman, J. J. Immunol. (1985) [Pubmed]
  16. Lymphocyte apoptosis: induction by gene transfer techniques. Ebert, O., Finke, S., Salahi, A., Herrmann, M., Trojaneck, B., Lefterova, P., Wagner, E., Kircheis, R., Huhn, D., Schriever, F., Schmidt-Wolf, I.G. Gene Ther. (1997) [Pubmed]
  17. Protein-tyrosine phosphatase-1B negatively regulates insulin signaling in l6 myocytes and Fao hepatoma cells. Egawa, K., Maegawa, H., Shimizu, S., Morino, K., Nishio, Y., Bryer-Ash, M., Cheung, A.T., Kolls, J.K., Kikkawa, R., Kashiwagi, A. J. Biol. Chem. (2001) [Pubmed]
  18. Transgenic rescue of Krabbe disease in the twitcher mouse. De Gasperi, R., Friedrich, V.L., Perez, G.M., Senturk, E., Wen, P.H., Kelley, K., Elder, G.A., Gama Sosa, M.A. Gene Ther. (2004) [Pubmed]
  19. Expression of the inhibitory Smad7 in early mouse development and upregulation during embryonic vasculogenesis. Zwijsen, A., van Rooijen, M.A., Goumans, M.J., Dewulf, N., Bosman, E.A., ten Dijke, P., Mummery, C.L., Huylebroeck, D. Dev. Dyn. (2000) [Pubmed]
  20. Analysis of hematopoietic stem cell reprogramming with toxigenicity. Marguerie, G., Tronik-Le Roux, D. Stem Cells (1998) [Pubmed]
  21. The ectopic study of tissue-engineered bone with hBMP-4 gene modified bone marrow stromal cells in rabbits. Jiang, X.Q., Chen, J.G., Gittens, S., Chen, C.J., Zhang, X.L., Zhang, Z.Y. Chin. Med. J. (2005) [Pubmed]
  22. Novel and effective gene transfer technique for study of vascular renin angiotensin system. Morishita, R., Gibbons, G.H., Kaneda, Y., Ogihara, T., Dzau, V.J. J. Clin. Invest. (1993) [Pubmed]
  23. Transfer of the bacterial gene for cytosine deaminase to mammalian cells confers lethal sensitivity to 5-fluorocytosine: a negative selection system. Mullen, C.A., Kilstrup, M., Blaese, R.M. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  24. Glucose sensing in pancreatic islet beta cells: the key role of glucokinase and the glycolytic intermediates. German, M.S. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  25. Distinct roles for amino- and carboxyl-terminal sequences of SPRR1 protein in the formation of cross-linked envelopes of conducting airway epithelial cells. Deng, J., Pan, R., Wu, R. J. Biol. Chem. (2000) [Pubmed]
  26. Protein kinase CbetaII regulates its own expression in rat intestinal epithelial cells and the colonic epithelium in vivo. Liu, Y., Su, W., Thompson, E.A., Leitges, M., Murray, N.R., Fields, A.P. J. Biol. Chem. (2004) [Pubmed]
  27. A modular cis-regulatory system controls isoform-specific pitx expression in ascidian stomodaeum. Christiaen, L., Bourrat, F., Joly, J.S. Dev. Biol. (2005) [Pubmed]
  28. Functional rescue of the glomerulosclerosis phenotype in Mpv17 mice by transgenesis with the human Mpv17 homologue. Schenkel, J., Zwacka, R.M., Rutenberg, C., Reuter, A., Waldherr, R., Weiher, H. Kidney Int. (1995) [Pubmed]
  29. Human and mouse ABCA1 comparative sequencing and transgenesis studies revealing novel regulatory sequences. Qiu, Y., Cavelier, L., Chiu, S., Yang, X., Rubin, E., Cheng, J.F. Genomics (2001) [Pubmed]
  30. A 178-kb BAC transgene imprints the mouse Gtl2 gene and localizes tissue-specific regulatory elements. Yevtodiyenko, A., Steshina, E.Y., Farner, S.C., Levorse, J.M., Schmidt, J.V. Genomics (2004) [Pubmed]
  31. Analysis of the Mom1 modifier of intestinal neoplasia in mice. Gould, K.A., Dove, W.F. Exp. Lung Res. (1998) [Pubmed]
  32. Coupled site-directed mutagenesis/transgenesis identifies important functional domains of the mouse agouti protein. Perry, W.L., Nakamura, T., Swing, D.A., Secrest, L., Eagleson, B., Hustad, C.M., Copeland, N.G., Jenkins, N.A. Genetics (1996) [Pubmed]
  33. Clonal segregation of oligodendrocytes and astrocytes during in vitro differentiation of glial progenitor cells. Lubetzki, C., Goujet-Zalc, C., Demerens, C., Danos, O., Zalc, B. Glia (1992) [Pubmed]
  34. APP transgenesis: approaches toward the development of animal models for Alzheimer disease neuropathology. Greenberg, B.D., Savage, M.J., Howland, D.S., Ali, S.M., Siedlak, S.L., Perry, G., Siman, R., Scott, R.W. Neurobiol. Aging (1996) [Pubmed]
  35. Transgenesis and gene targeting in the mouse; tools for studying genetic determinants of hypertension. Stec, D.E., Davisson, R.L., Sigmund, C.D. Trends Cardiovasc. Med. (1998) [Pubmed]
  36. Developmental regulation of the chicken delta 1-crystallin gene: analysis by transgenesis and gene dissection. Kondoh, H., Katoh, K., Takahashi, Y., Goto, K., Hayashi, S., Okada, T.S. Cell Differ. Dev. (1988) [Pubmed]
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