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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Cloning and alternative splicing analysis of Bombyx mori transformer-2 gene using silkworm EST database.

We have identified Bombyx mori transformer-2 gene (Bmtra-2) cDNA by blasting the EST database of B. mori. It was expressed in the whole life of the male and female silkworm and was observed as a band of 1.3 kb by Northern blot analysis. By comparing corresponding ESTs to the Bmtra-2 DNA sequence, it was revealed that there were eight exons and seven introns, and all splice sites of exons/introns conformed to the GT/AG rule. Bmtra-2 pre-mRNA can produce multiple mRNAs encoding six distinct isoforms of BmTRA-2 protein using an alternative splicing pathway during processing. Six types of Bmtra-2 cDNA clones were identified by reverse transcription-polymerase chain reaction. All isoforms of BmTRA-2 protein contain two arginine/serine-rich domains and one RNA recognition motif, showing striking organizational similarity to Drosophila TRA-2 proteins.[1]

References

  1. Cloning and alternative splicing analysis of Bombyx mori transformer-2 gene using silkworm EST database. Niu, B.L., Meng, Z.Q., Tao, Y.Z., Lu, S.L., Weng, H.B., He, L.H., Shen, W.F. Acta Biochim. Biophys. Sin. (Shanghai) (2005) [Pubmed]
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