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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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The cysteine-desulfurase IscS promotes the production of the rhodanese RhdA in the persulfurated form.

After heterologous expression in Escherichia coli, the Azotobacter vinelandii rhodanese RhdA is purified in a persulfurated form (RhdA-SSH). We identified l-cysteine as the most effective sulfur source in producing RhdA-SSH. An E. coli soluble extract was required for in vitro persulfuration of RhdA, and the addition of pyridoxal-5'-phosphate increased RhdA-SSH production, indicating a likely involvement of a cysteine desulfurase. We were able to show the formation of a covalent complex between IscS and RhdA. By combining a time-course fluorescence assay and mass spectrometry analysis, we demonstrated the transfer of sulfur from E. coli IscS to RhdA.[1]

References

  1. The cysteine-desulfurase IscS promotes the production of the rhodanese RhdA in the persulfurated form. Forlani, F., Cereda, A., Freuer, A., Nimtz, M., Leimkühler, S., Pagani, S. FEBS Lett. (2005) [Pubmed]
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