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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 
 

Retinal [125I]iodomelatonin binding sites in the tree shrew (Tupaiidae).

The characteristics of melatonin-binding sites labelled by [125I]iodomelatonin in membrane preparations from the tree shrew retina were determined. Specific binding of [125I]iodomelatonin to the membrane preparations of tree shrew retina was rapid, stable, saturable, and reversible. Among the indoles tested only 6-chloromelatonin, melatonin and N-acetylserotonin had significant affinities to the [125I]iodomelatonin binding site. Scatchard analysis of the membrane preparations revealed a dissociation constant (Kd) of 51.0 +/- 16 pM and a total number of binding sites (Bmax) of 1.97 +/- 0.6 fmol/mg protein.[1]

References

  1. Retinal [125I]iodomelatonin binding sites in the tree shrew (Tupaiidae). Lu, Y., Yuan, H., Pang, S.F. Neurosci. Lett. (1991) [Pubmed]
 
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