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Sanchez-Weatherby, J. et al.

Sanchez-Weatherby, Southall, Oubrie,

Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding.

The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described.[1]

References

Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding. Sanchez-Weatherby, J., Southall, S., Oubrie, A. Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. (2006) [Pubmed]

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