Ozone monitoring based on a biosensor concept utilizing a reagentless alcohol oxidase electrode.
An electrochemical method based on the concept of a biosensor for the monitoring of ozone is described for first time. The proposed method includes two parts: a selective sorbent for ozone, that is, eugenol, and a formaldehyde amperometric biosensor mounted into a flow-through cell. Ozone adds rapidly to the double bond of the allyl group of eugenol, which has been immobilized onto a hydrophobic C-18 reactor and the so produced formaldehyde is collected into the working buffer solution (sampler) and pumped to the detector. A multimembrane assembly consisting of an alcohol oxidase-modified nylon membrane sandwiched between an outer polycarbonate and an inner cellulose acetate membrane was fitted onto a Pt electrode and the enzymatically produced H(2)O(2) was monitored at +0.65 V (vs Ag/AgCl/KCl 3 M). Under optimum conditions, a linear calibration curve over the concentration range 3-200 microg x mL(-)(1) ozone was constructed. The detection limit (S/N = 3) was calculated at 1.1 microg x mL(-)(1) ozone. The proposed method is interference-free from other gases such as O(2), Ar, N(2), N(2)O, NOCl, SO(2), NH(3), and CO(2), which were tested at concentrations >200-fold higher than that of 100 microg x mL(-)(1) ozone used for comparison. Besides selectivity, the method is easy to perform and reproducible; its applicability in synthetic gaseous samples is also demonstrated.[1]References
- Ozone monitoring based on a biosensor concept utilizing a reagentless alcohol oxidase electrode. Stergiou, D.V., Prodromidis, M.I., Veltsistas, P.G., Evmiridis, N.P. Anal. Chem. (2006) [Pubmed]
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