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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Biosensing Techniques

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Disease relevance of Biosensing Techniques


Psychiatry related information on Biosensing Techniques

  • The biosensor operated at 0.450 V, had a fast response time (t90% < or = 3 s), and was free of typical interferences, and its dynamic range covered 3 orders of magnitude of glucose concentrations [6].

High impact information on Biosensing Techniques

  • Using a FRET biosensor, we find that LGN behaves as a conformational switch: in its closed state, the N and C termini interact, but NuMA or Galphai can disrupt this association, allowing LGN to interact simultaneously with both proteins, resulting in their cortical localization [7].
  • However, the adoption of biosensors for practical applications other than the measurement of blood glucose is currently limited by the expense, insensitivity and inflexibility of the available transduction methods [8].
  • Focal adhesion kinase (FAK) is a crucial signalling component that is activated by numerous stimuli and functions as a biosensor or integrator to control cell motility [9].
  • An emerging theme is that LIM proteins might function as biosensors that mediate communication between the cytosolic and the nuclear compartments [10].
  • Two-photon imaging reveals somatodendritic chloride gradient in retinal ON-type bipolar cells expressing the biosensor Clomeleon [11].

Chemical compound and disease context of Biosensing Techniques


Biological context of Biosensing Techniques

  • To demonstrate applicability to biosensor technology, we have introduced a series of point mutations in the maltose-binding site that lower the affinity of the protein for its ligand [17].
  • Here, we describe a GFP-based auxin biosensor to monitor auxin during Arabidopsis root gravitropism at cellular resolution [18].
  • Plasminogen bound to a site on the TF apoprotein that appears to be distinct from the binding site for factors VII and VIIa as judged by a combination of biosensor and cell assays [19].
  • Using a novel yellow fluorescent protein-based foldase biosensor, we demonstrated an upregulation of chaperone in situ activity in cells overexpressing full-length BAG1 but not in cells overexpressing BAGDeltaC compared to wild-type cells [20].
  • In the first, the sGM-CSFRalpha-Fc was bound to immobilized staphylococcal protein A on the biosensor surface, and binding kinetics of GM-CSF in solution were determined [21].

Anatomical context of Biosensing Techniques


Associations of Biosensing Techniques with chemical compounds

  • Using genetically targeted PIP2-sensitive inward rectifier channels (Kir2.1) as biosensors, we provide evidence that trp decay reflects depletion of PIP2 [27].
  • Iron-sulfur clusters as biosensors of oxidants and iron [28].
  • Here, we use "patch-cramming," in which an excised, inside-out membrane patch containing cyclic nucleotide-gated ion channels is used as a biosensor, to obtain the first real-time measurements of cGMP in intact cells [29].
  • In the present studies, a novel biosensor tool was engineered to directly determine whether MD cells release PGE2 in response to low luminal NaCl concentration ([NaCl]L) [30].
  • Conversion of a maltose receptor into a zinc biosensor by computational design [31].

Gene context of Biosensing Techniques

  • Using an optical biosensor, we found that CD4-induced (CD4i) epitopes recognized by mAbs 17b and 48d were more exposed on 8x than on IIIB gp120 [32].
  • Plasminogen was found to bind directly to the extracellular domain of TF apoprotein (amino acids 1-219) as determined by optical biosensor interaction analysis [19].
  • In this study we measured the high affinity binding of unlabeled IGFs and IGF analogues to recombinant unglycosylated IGFBP-3, using a BIAcoretrade mark instrument (Pharmacia Biosensor AB) [33].
  • Here we show, based on optical biosensor analyses, that immobilized HRG interacts with soluble plasminogen with high affinity and with an extremely slow dissociation rate [34].
  • To identify the domains involved in VEGF binding, we constructed various deletion mutants of the extracellular region fused with the crystallizable fragment portion of an IgG and then examined the binding affinity with VEGF by means of the BIAcore biosensor assay [35].

Analytical, diagnostic and therapeutic context of Biosensing Techniques


  1. Engineering regulable Escherichia coli beta-galactosidases as biosensors for anti-HIV antibody detection in human sera. Ferrer-Miralles, N., Feliu, J.X., Vandevuer, S., Müller, A., Cabrera-Crespo, J., Ortmans, I., Hoffmann, F., Cazorla, D., Rinas, U., Prévost, M., Villaverde, A. J. Biol. Chem. (2001) [Pubmed]
  2. Immobilized parathion hydrolase: an amperometric sensor for parathion. Sacks, V., Eshkenazi, I., Neufeld, T., Dosoretz, C., Rishpon, J. Anal. Chem. (2000) [Pubmed]
  3. High-resolution real-time recording with microelectrode biosensors reveals novel aspects of adenosine release during hypoxia in rat hippocampal slices. Frenguelli, B.G., Llaudet, E., Dale, N. J. Neurochem. (2003) [Pubmed]
  4. DNA biosensor for detection of Helicobacter pylori using phen-dione as the electrochemically active ligand in osmium complexes. del Pozo, M.V., Alonso, C., Pariente, F., Lorenzo, E. Anal. Chem. (2005) [Pubmed]
  5. Novel fluorescent biosensor for pathogenic toxins using cyclic polypeptide conjugates. Sadik, O.A., Yan, F. Chem. Commun. (Camb.) (2004) [Pubmed]
  6. Integration of enzymes and electrodes: spectroscopic and electrochemical studies of chitosan-enzyme films. Wei, X., Cruz, J., Gorski, W. Anal. Chem. (2002) [Pubmed]
  7. Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins. Du, Q., Macara, I.G. Cell (2004) [Pubmed]
  8. A biosensor that uses ion-channel switches. Cornell, B.A., Braach-Maksvytis, V.L., King, L.G., Osman, P.D., Raguse, B., Wieczorek, L., Pace, R.J. Nature (1997) [Pubmed]
  9. Focal adhesion kinase: in command and control of cell motility. Mitra, S.K., Hanson, D.A., Schlaepfer, D.D. Nat. Rev. Mol. Cell Biol. (2005) [Pubmed]
  10. The LIM domain: from the cytoskeleton to the nucleus. Kadrmas, J.L., Beckerle, M.C. Nat. Rev. Mol. Cell Biol. (2004) [Pubmed]
  11. Two-photon imaging reveals somatodendritic chloride gradient in retinal ON-type bipolar cells expressing the biosensor Clomeleon. Duebel, J., Haverkamp, S., Schleich, W., Feng, G., Augustine, G.J., Kuner, T., Euler, T. Neuron (2006) [Pubmed]
  12. A glucose oxidase electrode based on electropolymerized conducting polymer with polyanion-enzyme conjugated dopant. Sung, W.J., Bae, Y.H. Anal. Chem. (2000) [Pubmed]
  13. Hydrogel network entrapping cholesterol oxidase and octadecylsilica for optical biosensing in hydrophobic organic or aqueous micelle solvents. Wu, X.J., Choi, M.M. Anal. Chem. (2003) [Pubmed]
  14. Third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase. Stoica, L., Ludwig, R., Haltrich, D., Gorton, L. Anal. Chem. (2006) [Pubmed]
  15. General strategy for biosensor design and construction employing multifunctional surface-tethered components. Medintz, I.L., Anderson, G.P., Lassman, M.E., Goldman, E.R., Bettencourt, L.A., Mauro, J.M. Anal. Chem. (2004) [Pubmed]
  16. Identification of a quorum-sensing signal molecule in the facultative intracellular pathogen Brucella melitensis. Taminiau, B., Daykin, M., Swift, S., Boschiroli, M.L., Tibor, A., Lestrate, P., De Bolle, X., O'Callaghan, D., Williams, P., Letesson, J.J. Infect. Immun. (2002) [Pubmed]
  17. The rational design of allosteric interactions in a monomeric protein and its applications to the construction of biosensors. Marvin, J.S., Corcoran, E.E., Hattangadi, N.A., Zhang, J.V., Gere, S.A., Hellinga, H.W. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  18. Gravity-regulated differential auxin transport from columella to lateral root cap cells. Ottenschläger, I., Wolff, P., Wolverton, C., Bhalerao, R.P., Sandberg, G., Ishikawa, H., Evans, M., Palme, K. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  19. Tissue factor regulates plasminogen binding and activation. Fan, Z., Larson, P.J., Bognacki, J., Raghunath, P.N., Tomaszewski, J.E., Kuo, A., Canziani, G., Chaiken, I., Cines, D.B., Higazi, A.A. Blood (1998) [Pubmed]
  20. Interaction of BAG1 and Hsp70 mediates neuroprotectivity and increases chaperone activity. Liman, J., Ganesan, S., Dohm, C.P., Krajewski, S., Reed, J.C., Bähr, M., Wouters, F.S., Kermer, P. Mol. Cell. Biol. (2005) [Pubmed]
  21. Construction and binding kinetics of a soluble granulocyte-macrophage colony-stimulating factor receptor alpha-chain-Fc fusion protein. Monfardini, C., Ramamoorthy, M., Rosenbaum, H., Fang, Q., Godillot, P.A., Canziani, G., Chaiken, I.M., Williams, W.V. J. Biol. Chem. (1998) [Pubmed]
  22. A genetically engineered, protein-based optical biosensor of myosin II regulatory light chain phosphorylation. Post, P.L., Trybus, K.M., Taylor, D.L. J. Biol. Chem. (1994) [Pubmed]
  23. CX3CR1 tyrosine sulfation enhances fractalkine-induced cell adhesion. Fong, A.M., Alam, S.M., Imai, T., Haribabu, B., Patel, D.D. J. Biol. Chem. (2002) [Pubmed]
  24. Implanting the glucose enzyme electrode: problems, progress, and alternative solutions. Updike, S.J., Shults, M., Ekman, B. Diabetes Care (1982) [Pubmed]
  25. Evidence for a lactate pool in the rat brain that is not used as an energy supply under normoglycemic conditions. Leegsma-Vogt, G., Venema, K., Korf, J. J. Cereb. Blood Flow Metab. (2003) [Pubmed]
  26. Analysis for transaminases in serum with an amperometric glutamate electrode. Compagnone, D., Federici, G., Massoud, R., Santoro, L., Anichini, M., Palleschi, G. Clin. Chem. (1992) [Pubmed]
  27. Calcium influx via TRP channels is required to maintain PIP2 levels in Drosophila photoreceptors. Hardie, R.C., Raghu, P., Moore, S., Juusola, M., Baines, R.A., Sweeney, S.T. Neuron (2001) [Pubmed]
  28. Iron-sulfur clusters as biosensors of oxidants and iron. Rouault, T.A., Klausner, R.D. Trends Biochem. Sci. (1996) [Pubmed]
  29. Real-time patch-cram detection of intracellular cGMP reveals long-term suppression of responses to NO and muscarinic agonists. Trivedi, B., Kramer, R.H. Neuron (1998) [Pubmed]
  30. Luminal NaCl delivery regulates basolateral PGE2 release from macula densa cells. Peti-Peterdi, J., Komlosi, P., Fuson, A.L., Guan, Y., Schneider, A., Qi, Z., Redha, R., Rosivall, L., Breyer, M.D., Bell, P.D. J. Clin. Invest. (2003) [Pubmed]
  31. Conversion of a maltose receptor into a zinc biosensor by computational design. Marvin, J.S., Hellinga, H.W. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  32. Stable exposure of the coreceptor-binding site in a CD4-independent HIV-1 envelope protein. Hoffman, T.L., LaBranche, C.C., Zhang, W., Canziani, G., Robinson, J., Chaiken, I., Hoxie, J.A., Doms, R.W. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  33. Biosensor measurement of the binding of insulin-like growth factors I and II and their analogues to the insulin-like growth factor-binding protein-3. Heding, A., Gill, R., Ogawa, Y., De Meyts, P., Shymko, R.M. J. Biol. Chem. (1996) [Pubmed]
  34. Plasminogen is tethered with high affinity to the cell surface by the plasma protein, histidine-rich glycoprotein. Jones, A.L., Hulett, M.D., Altin, J.G., Hogg, P., Parish, C.R. J. Biol. Chem. (2004) [Pubmed]
  35. Mapping of the sites involved in ligand association and dissociation at the extracellular domain of the kinase insert domain-containing receptor for vascular endothelial growth factor. Shinkai, A., Ito, M., Anazawa, H., Yamaguchi, S., Shitara, K., Shibuya, M. J. Biol. Chem. (1998) [Pubmed]
  36. Kinetic analysis of estrogen receptor/ligand interactions. Rich, R.L., Hoth, L.R., Geoghegan, K.F., Brown, T.A., LeMotte, P.K., Simons, S.P., Hensley, P., Myszka, D.G. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  37. Identification of the heparan sulfate binding sites in the cellular prion protein. Warner, R.G., Hundt, C., Weiss, S., Turnbull, J.E. J. Biol. Chem. (2002) [Pubmed]
  38. Biophysical characterization of interactions involving importin-alpha during nuclear import. Catimel, B., Teh, T., Fontes, M.R., Jennings, I.G., Jans, D.A., Howlett, G.J., Nice, E.C., Kobe, B. J. Biol. Chem. (2001) [Pubmed]
  39. Purification and characterization of a novel restricted antigen expressed by normal and transformed human colonic epithelium. Catimel, B., Ritter, G., Welt, S., Old, L.J., Cohen, L., Nerrie, M.A., White, S.J., Heath, J.K., Demediuk, B., Domagala, T., Lee, F.T., Scott, A.M., Tu, G.F., Ji, H., Moritz, R.L., Simpson, R.J., Burgess, A.W., Nice, E.C. J. Biol. Chem. (1996) [Pubmed]
  40. Heterogeneous nuclear ribonucleoprotein A3, a novel RNA trafficking response element-binding protein. Ma, A.S., Moran-Jones, K., Shan, J., Munro, T.P., Snee, M.J., Hoek, K.S., Smith, R. J. Biol. Chem. (2002) [Pubmed]
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