Molecular cloning and sequencing of genomic DNA encoding yeast vacuolar carboxypeptidase yscS.
A Saccharomyces cerevisiae genomic DNA encoding vacuolar carboxypeptidase yscS was cloned from a yeast YEp13 library by complementation of the previously characterized mutation cps1-1 [(1981) J. Bacteriol. 147, 418-426], by means of staining carboxypeptidase activity in yeast colonies. The nucleotide sequence of the cloned gene was determined. The open reading frame of CPS1 consists of 576 codons and therefore encodes a protein of 64961 molecular weight. A stretch of 19 residues near the N-terminus of the deduced polypeptide sequence contains characteristics common to known hydrophobic leader sequences. CPS1 was determined by DNA blot analysis to be a single copy gene located on chromosome X. The cloned fragment was used to identify a 2.1 kb mRNA. A transcriptional activation of CPS1 occurs when cells grow on a substrate of carboxy-peptidase yscS as sole nitrogen source.[1]References
- Molecular cloning and sequencing of genomic DNA encoding yeast vacuolar carboxypeptidase yscS. Bordallo, J., Bordallo, C., Gascón, S., Suárez-Rendueles, P. FEBS Lett. (1991) [Pubmed]
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