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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats.

The distribution of alcohol dehydrogenase in the hepatic acinus was examined by both histochemical and immunohistochemical approaches. The immunohistochemical method using anti-alcohol dehydrogenase antibody indicated zone 3 predominance of this enzyme in the hepatic acinus, whereas a conventional histochemical method showed slight zone 1 predominance. However, when the histochemical technique was improved by using 2% glutaraldehyde instead of formalin for fixation and by adding phenazine methosulfate (0.33 mmol/L) to the staining incubation mixture, this method also supported zone 3 predominance of alcohol dehydrogenase. Evidence for zone 3 distribution of alcohol dehydrogenase may be of value in elucidating the mechanism of zone 3 liver damage by alcohol.[1]

References

  1. Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats. Kato, S., Ishii, H., Aiso, S., Yamashita, S., Ito, D., Tsuchiya, M. Hepatology (1990) [Pubmed]
 
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