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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Metabolic stress in isolated mouse ventricular myocytes leads to remodeling of t tubules.

Cardiac ventricular myocytes possess an extensive t-tubular system that facilitates the propagation of membrane potential across the cell body. It is well established that ionic currents at the restricted t-tubular space may lead to significant changes in ion concentrations, which, in turn, may affect t-tubular membrane potential. In this study, we used the whole cell patch-clamp technique to study accumulation and depletion of t-tubular potassium by measuring inward rectifier potassium tail currents (I(K1,tail)), and inward rectifier potassium current (I(K1)) "inactivation". At room temperatures and in the absence of Mg(2+) ions in pipette solution, the amplitude of I(K1,tail) measured ∼10 min after the establishment of whole cell configuration was reduced by ∼18%, but declined nearly twofold in the presence of 1 mM cyanide. At ∼35°C I(K1,tail) was essentially preserved in intact cells, but its amplitude declined by ∼85% within 5 min of cell dialysis, even in the absence of cyanide. Intracellular Mg(2+) ions played protective role at all temperatures. Decline of I(K1,tail) was accompanied by characteristic changes in its kinetics, as well as by changes in the kinetics of I(K1) inactivation, a marker of depletion of t-tubular K(+). The data point to remodeling of t tubules as the primary reason for the observed effects. Consistent with this, detubulation of myocytes using formamide-induced osmotic stress significantly reduced I(K1,tail), as well as the inactivation of inward I(K1). Overall, the data provide strong evidence that changes in t tubule volume/structure may occur on a short time scale in response to various types of stress.[1]


  1. Metabolic stress in isolated mouse ventricular myocytes leads to remodeling of t tubules. Cheng, L.F., Wang, F., Lopatin, A.N. Am. J. Physiol. Heart Circ. Physiol. (2011) [Pubmed]
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