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MeSH Review

Patch-Clamp Techniques

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Disease relevance of Patch-Clamp Techniques


High impact information on Patch-Clamp Techniques


Chemical compound and disease context of Patch-Clamp Techniques


Biological context of Patch-Clamp Techniques


Anatomical context of Patch-Clamp Techniques


Associations of Patch-Clamp Techniques with chemical compounds

  • Present thinking about the way that the NMDA (N-methyl-D-aspartate) class of glutamate receptor operates at central synapses relies mainly on information obtained from single-channel and whole-cell recordings from cultured neurons stimulated by exogenous NMDA receptor agonists [26].
  • By analysing the responses of mouse central neurones to glutamate using the patch-clamp technique, we have now found a link between voltage sensitivity and Mg2+ sensitivity [27].
  • To investigate the membrane effects of noradrenaline on central neurons, we used a newly developed preparation in which patch-clamp techniques can be applied to exposed adult cortical neurons [28].
  • We report here that application of the patch-clamp technique to CN-treated mammalian heart cells reveals specific K+ channels which are depressed by intracellular ATP (ATPi) at levels greater than 1 mM [29].
  • In order to determine whether inhibitory inputs are necessary for a single cortical neuron to show orientation selectivity, GABA receptors were blocked intracellularly during whole cell recording [30].

Gene context of Patch-Clamp Techniques

  • METHODS: The patch-clamp technique for whole-cell recording was used in cultured or single interstitial cells of Cajal. TRPM7-specific small interfering RNAs were used for specific inhibition of TRPM7 [31].
  • In the present study we examined in detail the possibility that BDNF modulates synaptic neurotransmissions by using patch-clamp technique in rat hippocampal CA1 region [32].
  • This specific localization was supported by measuring the magnitude of a TRP-dependent inward current that results from spontaneous activation of the light-sensitive channels during whole-cell recordings (the rundown current, RDC) [33].
  • We have investigated whether changes in the extracellular anionic environment affects the activity of CFTR using the patch clamp technique [34].
  • Whole cell recordings from non-transfected HEK cells and cells expressing human TRPV6 revealed the presence of a basal inward current in both types of cells when the internal solution contained 0.1 mm EGTA and 100 nm [Ca(2+)](i) or if the cytosolic Ca(2+) buffering remained undisturbed in perforated patch-clamp experiments [35].

Analytical, diagnostic and therapeutic context of Patch-Clamp Techniques


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