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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Circular DNA is excised by immunoglobulin class switch recombination.

We have purified extrachromosomal circular DNAs from adult mouse spleen cells, and cloned into a phage vector the BamHl fragments hybridizing with C mu and S gamma 1 probes. We obtained 52 S mu+S gamma 1+ clones by screening 1.4 million phage clones derived from spleen cells stimulated with bacterial lipopolysaccharide and interleukin 4. We have identified the breakpoints of six clones that contain S gamma 1 and S mu sequences fused in the 5' to 3' orientation. All these switch recombination sites were assigned to the central repetitive sequences of the S mu and S gamma 1 regions. Since the common S mu-S gamma 1 sequences at the recombination sites are at most 2 bases long, typical homologous recombination cannot account for their joining. These findings provide direct evidence that mu-gamma 1 class switching can occur by the looping out and excision of chromosomal DNA, with formation of a circle.[1]


  1. Circular DNA is excised by immunoglobulin class switch recombination. Iwasato, T., Shimizu, A., Honjo, T., Yamagishi, H. Cell (1990) [Pubmed]
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