Divergent effects of insulin on insulin-like growth factor-II gene expression in the rat hypothalamus.
The effect of peripheral insulin treatment on brain content and synthesis of insulin-like growth factor-II (IGF-II) was studied in acute and chronic conditions. After a 3-h hyperinsulinemic/euglycemic clamp, rats were killed, and brains were removed for IGF-II analysis. Insulin infusion elevated IGF-II concentrations in the dorsomedial hypothalamus (DMH) and suprachiasmatic nucleus, but decreased IGF-II in the paraventricular nucleus, lateral hypothalamus (LtH), and supraoptic nucleus compared to values in control animals. In separate parallel studies, the effects of 4 days of insulin injection (1 U insulin/rat, sc, twice daily) on brain IGF-II mRNA and peptide were determined. For RNA analysis the hypothalamus was divided into three regions (lateral, ventral, and dorsal). IGF-II mRNA content was shown to vary by an order of magnitude within the adult rat hypothalamus under control conditions, and there appeared to be a differential region-dependent response to insulin treatment. Relative abundance of IGF-II mRNA in control hypothalami was VH much greater than cortex greater than or equal to LtH greater than DMH. IGF-II mRNA content declined after insulin treatment in the VH, but rose in all other regions. Insulin increased IGF-II peptide content in the paraventricular nucleus, LtH, and VMH but decreased IGF-II in the DMH and suprachiasmatic nucleus. These results indicate that peripheral insulin status may be an important factor in the synthesis and secretion of IGF-II in the brain.[1]References
- Divergent effects of insulin on insulin-like growth factor-II gene expression in the rat hypothalamus. Lauterio, T.J., Aravich, P.F., Rotwein, P. Endocrinology (1990) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg