Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Ivanov, E.L. et al.

The rad2 mutation affects the molecular nature of UV and acridine-mustard- induced mutations in the ADE2 gene of Saccharomyces cerevisiae.

We have studied the molecular nature of ade2 mutations induced by UV light and bifunctional acridine-mustard (BAM) in wild-type (RAD) and in excision-deficient (rad2) strains of the yeast, Saccharomyces cerevisiae. In the RAD strain, UV causes 45% GC----AT transitions among all mutations; in the rad2 strain this value is 77%. BAM was shown to be highly specific for frameshift mutagenesis: 60% frameshifts in the RAD strain, and as many as 84% frameshifts in the rad2 strain were induced. Therefore, the rad2 mutation affects the specificity of UV- and BAM-induced mutagenesis in yeast. Experimental data agree with the view that the majority of mutations in the RAD strain are induced by a prereplicative mechanism, whereas mutations in the RAD strain are induced by a prereplicative mechanism, whereas mutations in the rad2 strain are predominantly postreplicative events. Our results also suggest that: cytosine-containing photoproducts are the substances responsible for major premutational damage to cytosine-containing photoproducts are the substances responsible for major premutational damage to DNA; a fraction of the mutations may arise in the course of excision repair of UV photoproducts.[1]

References

The rad2 mutation affects the molecular nature of UV and acridine-mustard-induced mutations in the ADE2 gene of Saccharomyces cerevisiae. Ivanov, E.L., Kovaltzova, S.V., Kassinova, G.V., Gracheva, L.M., Korolev, V.G., Zakharov, I.A. Mutat. Res. (1986) [Pubmed]

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