Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Kley, N. et al.

Proenkephalin A gene expression in bovine adrenal chromaffin cells is regulated by changes in electrical activity.

Concentrations of mRNA coding for the opioid peptide precursor proenkephalin A (mRNAENK) were measured in primary cultures of bovine adrenal chromaffin cells maintained in serum-free medium. Using a sensitive solution hybridization assay, an increase in mRNAENK levels from 45 to 300% above control with K+ (10-20 mM), Ba2+ (1 mM) and veratridine (5 microM) was found. The highest increase (300% above control) was obtained with the Na+ channel agonist veratridine. This effect was nearly abolished in the presence of the Na+ channel antagonist tetrodotoxin (TTX) (1 microM). Moreover, TTX partially inhibited the increase in mRNAENK levels caused by K+ (20 mM) depolarization (from 185 to 130% of control), but had no effect on the stimulation by Ba2+ (1 mM). The Ca2+ channel antagonists D600 (50 microM) verapamil (50 microM) and Co2+ (1 mM) inhibited the responses to either K+, Ba2+ or veratridine, whereas the Ca2+ channel agonist Bay K 8644 (0.1 microM) potentiated the effect of 20 mM K+ from 185 to 230% of control. The K+-induced increase in the mRNAENK levels was associated with an increase of immunoreactive proenkephalin A-derived peptides in both tissue and medium, indicating an enhanced production of opioid peptides. These results suggest that membrane depolarization may play an important role in the regulation of proenkephalin A gene expression in bovine adrenal chromaffin cells. It may represent a mode by which substances acting directly on Na+ or Ca2+ channels may modulate the regulation of proenkephalin A mRNA biosynthesis and opioid peptide production.[1]

References

Proenkephalin A gene expression in bovine adrenal chromaffin cells is regulated by changes in electrical activity. Kley, N., Loeffler, J.P., Pittius, C.W., Höllt, V. EMBO J. (1986) [Pubmed]

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