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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Rapid identification of penicillinase-producing Neisseria gonorrhoeae by detection of beta-lactamase in urethral exudates.

The production of fluorescent end-products with ampicillin as substrate can be used to detect beta-lactamase activity in biological fluids. A fluorescent spot test was evaluated as a rapid method of detecting beta-lactamase in the urethral exudates of men infected with Neisseria gonorrhoeae in Thailand. Among 208 men with culture-proven gonococcal urethritis, the fluorescent spot test was positive in 92 of 101 men from whom penicillinase-producing N gonorrhoeae (PPNG) were isolated and in 4 of 107 men from whom non-PPNG were isolated. The fluorescent spot test is a rapid, inexpensive, and sensitive method to detect PPNG and may be valuable in determining appropriate treatment for patients with gonococcal urethritis.[1]

References

  1. Rapid identification of penicillinase-producing Neisseria gonorrhoeae by detection of beta-lactamase in urethral exudates. Taylor, D.N., Chen, K.C., Panikabutra, K., Wongba, C., Chitwarakern, A., Echeverria, P., Holmes, K.K. Lancet (1985) [Pubmed]
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