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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Anti-OTF-1 antibodies inhibit NFIII stimulation of in vitro adenovirus DNA replication.

HeLa cell OTF-1 has been purified on the basis of its DNA binding activity and used to raise a polyclonal rabbit antiserum. This antiserum is shown to recognize both native and denatured OTF-1 from both human and a similar protein from Xenopus culture cells, but to react either more weakly or not at all with the lymphoid cell-specific OTF-2. Separately, NFIII has been purified on the basis of its ability to stimulate Adenovirus DNA replication in vitro. On denaturing polyacrylamide gels OTF-1 and NFIII exhibit identical mobility. Anti-OTF-1 antiserum recognizes NFIII and neutralizes its stimulatory effect on DNA replication. Moreover, OTF-1 can functionally replace NFIII. Taken together with previously published DNA binding data, this indicates that OTF-1 and NFIII are either very closely related or identical.[1]

References

  1. Anti-OTF-1 antibodies inhibit NFIII stimulation of in vitro adenovirus DNA replication. Pruijn, J.M., van der Vliet, P.C., Dathan, N.A., Mattaj, I.W. Nucleic Acids Res. (1989) [Pubmed]
 
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