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Beck, W.D. et al.

Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA.

Additional DNA was shown to be present in methicillin-resistant Staphylococcus aureus by one- and two-dimensional restriction endonuclease analyses of the chromosomal DNA. A 3.5-kilobase Bg/II fragment, which was present in methicillin-resistant strains but not in the isogenic methicillin-sensitive parental strain, was cloned into newly constructed plasmid pWDB1 in Escherichia coli. Hybridization of this 3.5-kilobase Bg/II fragment with different methicillin-sensitive and methicillin-resistant S. aureus clinical isolates indicated that the fragment represents part of the methicillin resistance determinant ( mec). In addition, the fragment carries a sequence that is present in some large staphylococcal plasmids, as well as in penicillinase plasmid pI524.[1]

References

Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA. Beck, W.D., Berger-Bächi, B., Kayser, F.H. J. Bacteriol. (1986) [Pubmed]

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