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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Nerve growth factor ( NGF) is present in human placenta and semen, but undetectable in normal and Paget's disease blood: measurements with an anti-mouse- NGF enzyme immunoassay using a recombinant human NGF reference.

An enzyme immunoassay (EIA) originally developed for mouse beta-nerve growth factor ( NGF) and commercially available was validated for human NGF. Cell culture medium containing bioactive recombinant human NGF was used as a reference, and mouse 2.5S beta- NGF as a standard. One of three human placentas contained measurable NGF (70 pg/g of tissue of mouse beta- NGF equivalents), a second detectable, and a third undetectable NGF. In three human semen samples NGF content ranged from 0.13 - 1.4 ng/ml. NGF could not be detected in normal human serum and in plasma from patients with Paget's disease, although mouse 2.5S beta- NGF added to human blood could be completely recovered from the serum.[1]

References

  1. Nerve growth factor (NGF) is present in human placenta and semen, but undetectable in normal and Paget's disease blood: measurements with an anti-mouse-NGF enzyme immunoassay using a recombinant human NGF reference. Heinrich, G., Meyer, T.E. Biochem. Biophys. Res. Commun. (1988) [Pubmed]
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