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MeSH Review

Cell Culture Techniques

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Disease relevance of Cell Culture Techniques


Psychiatry related information on Cell Culture Techniques


High impact information on Cell Culture Techniques

  • In rheumatoid joint cell cultures that spontaneously produce IL-1, TNF alpha was the major dominant regulator of IL-1 [11].
  • Often, in cells grown in cell culture, drug resistance correlates with the presence of one or more of the so-called P-glycoproteins or multidrug resistance proteins, products of the mdr family of genes [12].
  • Using a mammalian cell culture system, we demonstrate that recycling endosomes are essential for Delta activity [13].
  • Furthermore, loss of dynein function resulted in premature aggregate formation by mutant huntingtin and increased levels of the autophagosome marker LC3-II in both cell culture and mouse models, compatible with impaired autophagosome-lysosome fusion [14].
  • Recent work using three-dimensional cell culture models underscores the importance of the interactions between epithelial cells-both normal and malignant-and the extracellular matrix that surrounds them in informing life and death decisions [15].

Chemical compound and disease context of Cell Culture Techniques


Biological context of Cell Culture Techniques

  • In many human cancers and transformed cell lines, Stat3 is persistently activated, and in cell culture, active Stat3 is either required for transformation, enhances transformation, or blocks apoptosis [20].
  • Heat-shock-induced alterations of ribosomal protein phosphorylation in plant cell cultures [21].
  • MEIS1 is able to rescue hth mutant phenotypes and can induce the cytoplasmic-to-nuclear translocation of EXD in cell culture and Drosophila embryos [22].
  • Telomere shortening in human and mouse tissues and primary cell cultures may be due to the absence of telomerase activity [23].
  • As demonstrated here, two polyadenylated jockey transcripts detected at different stages of Drosophila ontogenesis and in cell cultures have the same length as genomic copies of jockey and correspond to the strand containing ORFs. alpha-amanitin experiments indicate that jockey is transcribed by RNA polymerase II [24].

Anatomical context of Cell Culture Techniques


Associations of Cell Culture Techniques with chemical compounds


Gene context of Cell Culture Techniques

  • Recombinant TGF-beta1 stimulated Abeta clearance in microglial cell cultures [35].
  • Here we show that tPA upregulates MMP-9 in cell culture and in vivo [36].
  • We demonstrate this specific cleavage in cell culture, in neonatal mouse skin and with recombinant Dsg1, and conclude that Dsg1 is the specific receptor for exfoliative toxin A cleavage [37].
  • Here we have established cell cultures from the brain of an individual with VWM carrying mutations in subunit 5 of eIF2B (encoded by EIF2B5) [38].
  • RANTES and other chemokines that interact with CCR5 and block infection of peripheral blood mononuclear cell cultures inhibit infection of primary macrophages inefficiently at best [39].

Analytical, diagnostic and therapeutic context of Cell Culture Techniques

  • I report here that in the dispersed cell culture of chicken pineal gland, N-acetyltransferase activity exhibits a circadian rhythm and responds to environmental lighting in the same manner as in the organ culture [40].
  • These secreted vesicles, named exosomes, were isolated from the cell culture media by differential centrifugation followed by flotation on sucrose density gradients [41].
  • Messenger RNA from adult rat anterior pituitary fragments and cell cultures was reverse transcribed and subjected to PCR amplification using primers specific to the rat CRH gene [42].
  • COX-2 expression and activity were determined on pooled cell cultures by reverse transcription-polymerase chain reaction and prostaglandin E(2) (PGE(2)) enzyme immunoassay, respectively [43].
  • Twenty-five volunteers were randomly assigned to subcutaneous injections of the recombinant (three groups [A, B, and C] received two doses each of 10(3.5), 10(4.5), and 10(5.5) tissue-culture infectious doses50, respectively) or of human diploid cell culture vaccine (HDC; 6.52 international potency units per dose) [44].


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  13. Asymmetric Rab 11 endosomes regulate delta recycling and specify cell fate in the Drosophila nervous system. Emery, G., Hutterer, A., Berdnik, D., Mayer, B., Wirtz-Peitz, F., Gaitan, M.G., Knoblich, J.A. Cell (2005) [Pubmed]
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  17. Expression and localization of endothelin-1 and endothelin receptors in human meningiomas. Evidence for a role in tumoral growth. Pagotto, U., Arzberger, T., Hopfner, U., Sauer, J., Renner, U., Newton, C.J., Lange, M., Uhl, E., Weindl, A., Stalla, G.K. J. Clin. Invest. (1995) [Pubmed]
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  23. Differential regulation of telomerase activity and telomerase RNA during multi-stage tumorigenesis. Blasco, M.A., Rizen, M., Greider, C.W., Hanahan, D. Nat. Genet. (1996) [Pubmed]
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