The Ro/ SSA autoantigen as an immunogen. Some anti-Ro/ SSA antibody binds IgG.
The rheumatic disease autoantigen, Ro/ SSA, was immunogenic to a rabbit host. The heteroimmune rabbit serum bound the Ro/ SSA particle in immunoblots and in an ELISA. Both the rabbit anti-Ro/SSA and a human prototype anti-Ro/ SSA serum also bound IgG; and moreover, IgG inhibited both rabbit and human anti-Ro/ SSA activity. Anti-IgG activity of the rabbit and human anti-Ro/ SSA sera bound Ro/ SSA by Western blot and solid-phase assays. In addition, purified Ro/ SSA inhibited the anti-IgG activity of the anti-Ro/ SSA sera from rabbit and man. Affinity purification of the IgG- and Ro/ SSA-binding fractions of the rabbit anti-Ro/SSA demonstrated that both the anti-Ro/ SSA and anti-IgG activities were concentrated in these fractions. These data show that Ro/ SSA and IgG share epitopes that are bound by anti-Ro/ SSA antibody. Inhibition experiments suggest that this antibody is found in most human anti-Ro/ SSA autoimmune sera and that the epitope(s) are found in the F(ab')2 fragment of IgG.[1]References
- The Ro/SSA autoantigen as an immunogen. Some anti-Ro/SSA antibody binds IgG. Mamula, M.J., Fox, O.F., Yamagata, H., Harley, J.B. J. Exp. Med. (1986) [Pubmed]
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