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Phosphorylation of actin-binding proteins by casein kinases 1 and 2.

Filamin and vinculin from chicken gizzards were significantly phosphorylated in vitro by casein kinases 1 and 2, but not by alpha-actinin. Antisera raised against these actin-binding proteins immunoprecipitated the phosphorylated proteins corresponding to filamin and vinculin, but no phosphoprotein corresponding to alpha-actinin was detected. These results suggest that filamin and vinculin are phosphorylated in vivo but alpha-actinin is not.[1]


  1. Phosphorylation of actin-binding proteins by casein kinases 1 and 2. Nakajo, S., Nakaya, K., Nakamura, Y. Biochem. Int. (1987) [Pubmed]
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