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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Detection of Gardnerella vaginalis in vaginal specimens by direct immunofluorescence.

The preparation of a fluorescein-labeled Gardnerella vaginalis polyclonal antibody is described, and its usefulness is assessed for the detection of this microorganism in vaginal samples obtained from 263 women attending the gynecological department of a general hospital, 66 of whom harbored an intrauterine device. The direct immunofluorescence technique was positive for G. vaginalis in 21% of the specimens, whereas only 12.5% of the total bacteriological cultures were positive. The frequency was higher in patients harboring intrauterine devices since 34.8% exhibited positive immunofluorescence and 30.3% exhibited positive cultures.[1]


  1. Detection of Gardnerella vaginalis in vaginal specimens by direct immunofluorescence. Hansen, W., Vray, B., Miller, K., Crokaert, F., Yourassowsky, E. J. Clin. Microbiol. (1987) [Pubmed]
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