Rat cellular retinol-binding protein: cDNA sequence and rapid retinol-dependent accumulation of mRNA.
Cellular retinol-binding protein ( CRBP) may be an important mediator of vitamin A action. We report here the identification of a cDNA clone corresponding to the rat CRBP gene. The cDNA is 695 nucleotides long, with an open reading frame corresponding to a protein of 134 amino acids. The deduced amino acid sequence is identical with that of rat CRBP. The nucleotide sequence shows 90.5% similarity with the human CRBP cDNA sequence. Genomic DNA analysis indicates that CRBP is present in one, or at most two, copies within the rat genome. Analysis of mRNA reveals a single species in every tissue tested and suggests that the isolated cDNA is full-length. Finally, when retinol-deficient rats are fed retinyl acetate for 4 hr, about 4-fold accumulation of CRBP-specific mRNA is observed in the lungs. This rapid effect suggests that the micronutrient retinol may directly influence the expression of its specific intracellular binding protein.[1]References
- Rat cellular retinol-binding protein: cDNA sequence and rapid retinol-dependent accumulation of mRNA. Sherman, D.R., Lloyd, R.S., Chytil, F. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
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