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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Mucin-carbohydrate directed monoclonal antibody.

To raise monoclonal antibodies recognizing cancer-associated alterations of the carbohydrate structure of glycoproteins, Balb/c mice were immunized with human colonic cancer cells (LS 180 from ATCC). One of the generated hybridomas produced a monoclonal antibody that bound to the carbohydrate moiety of mucin-type glycoproteins from LS 180. The antibody did not bind to glycoproteins from another colonic cancer cell line, SW 1116, or to glycolipids from any of the colonic cancer cell lines. The antibody bound to ovine and bovine submaxillary mucins ( OSM and BSM). NeuAc alpha 2----6Ga1NAc seemed to be involved in the epitope.[1]

References

  1. Mucin-carbohydrate directed monoclonal antibody. Kurosaka, A., Fukui, S., Kitagawa, H., Nakada, H., Numata, Y., Funakoshi, I., Kawasaki, T., Yamashina, I. FEBS Lett. (1987) [Pubmed]
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