The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Transfer of mannose from GDP-mannose to lipid-linked oligosaccharide by soluble mannosyl transferase.

A solubilized mannosyl transferase(s) was obtained by treatment of the pig aorta particulate enzyme with the nonionic detergent Nonidet P-40 followed by centrifugation at 100,000 X g for 60 min. This enzyme preparation catalyzed the transfer of mannose from GDP-[14C]mannose (but not from [14C]mannosylphosphoryldolichol) to form a heptasaccharide-lipid. The synthesis of this heptasaccharide-lipid required the addition of an acceptor lipid that was isolated from pig liver. The oligosaccharide portion of the acceptor lipid appeared to be a mixture of trisaccharide and pentasaccharide. The formation of heptasaccharide-lipid did not require divalent cation, was not inhibited by EDTA, and was not inhibited by the antibiotic amphomycin. The heptasaccharide portion of the heptasaccharide-lipid had the same migration properties on paper chromatograms in two different solvent systems as a previously characterized Man5GlcNAc2 oligosaccharide [Li, E. & Kornfeld, S. (1979) J. Biol. Chem. 254, 2754-2758]. It also had the same migration properties as the oligosaccharide that accumulates in the presence of amphomycin. All three of these oligosaccharides emerged from a Bio-Gel P-4 column in the same position. Radioactive mannose was released from the heptasaccharide by digestion with alpha-mannosidase. These data demonstrate that at least some of the alpha-linked mannose residues in the heptasaccharide-lipid are donated directly from GDP-mannose.[1]


WikiGenes - Universities