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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Purification of retinal S-antigen to homogeneity by the criterion of gel electrophoresis silver staining.

This report describes a procedure by which high performance liquid chromatographic (HPLC) techniques are used to obtain homogeneous S-antigen. Conventionally prepared S-antigen was purified further by fractionation on the anion exchange Mono-Q column followed by gel filtration on a TSK-3000 column. This procedure produced an S-antigen preparation, which appeared homogeneous by gel electrophoresis using the very sensitive silver staining method. The purified material retained its immunochemical and uveitogenic activity. The availability of homogeneous S-antigen will facilitate studies aimed at elucidating, at the molecular level, the mechanism by which S-antigen induces uveitis.[1]

References

  1. Purification of retinal S-antigen to homogeneity by the criterion of gel electrophoresis silver staining. Zigler, J.S., Mochizuki, M., Kuwabara, T., Gery, I. Invest. Ophthalmol. Vis. Sci. (1984) [Pubmed]
 
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