The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Use of chromatofocusing to distinguish estradiol receptor from ovarian-dependent and -independent rat mammary tumors.

Cytosol estradiol receptor from MTW9-D (ovarian dependent) and MTW9-MtT (ovarian independent) rat mammary tumors were fractionated by chromatofocusing, a procedure which separates proteins on an ion-exchange column as a function of isoelectric point. Receptor from MTW9-D usually fractionated as three peaks with mean pH at peak height of 7.5, 6.8, and 6. 0. The intermediate peak at pH 6.8 was present in 90% of MTW9-D tumors examined but in only 20% of MTW9-MtT tumors. Treatment of cytosols with 20 mM sodium molybdate or 50 mM leupeptin did not change the chromatofocusing profiles. The pI 6.8 fraction of receptor bound quantitatively to DNA-cellulose after ammonium sulfate precipitation, while receptor in the other two peaks bound much less. The quantitative binding of a fraction of estradiol receptor characteristic of MTW9-D to DNA is consistent with the greater binding of unfractionated cytosol receptor from MTW9-D to DNA than the binding of receptor from MTW9-MtT. Sucrose gradient analysis of the pI 6.8 receptor showed a sedimentation coefficient slightly less than ovalbumin with a Stokes radius of 26 A as determined by agarose chromatography. The correlation of receptor binding to DNA with response to ovariectomy might make this form of receptor a potential marker of hormonal responsivity in mammary tumors.[1]

References

  1. Use of chromatofocusing to distinguish estradiol receptor from ovarian-dependent and -independent rat mammary tumors. Coufalik, A.H., Feldman, M., Platica, M., Toth, L., Dreiling, D., Hollander, V.P. Cancer Res. (1983) [Pubmed]
 
WikiGenes - Universities