Disease relevance of CCRIS 3927

• DCC assay and its modified procedures including 5% DCC stripping of cytosol and/or the addition of sodium molybdate in buffer were simultaneously measured in 5 gastric adenocarcinoma tissues and 1 gastritis cystica polyposa tissue (a precancerous lesion) [1].
• Since accurate androgen receptor assays could be valuable for therapeutic decision making in prostate cancer, we advocate the inclusion of sodium molybdate in assay buffers to guard against the possibility of false negative or artificially low values [2].
• Based on the results of a toxicity study of sodium molybdate, two doses, 200 and 400 mg/kg, were assessed in the bone marrow MN assay in mice (two i.p. injections 24 and 48 hr prior to euthanasia) [3].
• The addition of sodium molybdate (20 mM) to human breast tumor homogenates results in an increased yield of estrogen receptor sites in the cytosol, but reduces the number of sites detectable in salt extracts of purified nuclei [4].
• Sodium molybdate was administered orally to adult male rat at dose level of 10, 30, and 50 mg kg body weight (5 days per week) for 60 days [5].

High impact information on CCRIS 3927

• Dihydrotestosterone-receptor complexes formed in human fibroblast cytosol prepared at 0 degrees C in the presence of sodium molybdate can be readily transformed to the DNA-binding state by heating at 25 degrees C. Under these conditions 50-70% of dihydrotestosterone-receptor complexes bind to DNA [6].
• We analyzed two aspects of the radioligand interaction; nuclear uptake with dispersed, intact cells at 37 degrees C and binding at 0 degrees C with soluble extract ("cytosol") prepared from cells disrupted in buffer containing 300 mM KCl and 10 mM sodium molybdate [7].
• Using the DCC procedure with addition of sodium molybdate in buffer for receptor analysis, PgR and ER were found in gastric tissues in six patients, with significantly increased levels of measurable PgR [1].
• These results suggest that the mechanism of action for modulator is identical to that of sodium molybdate, and we propose that modulator is the endogenous molybdate factor for the glucocorticoid receptor [8].
• Sodium molybdate, which blocks the thermal activation step, inhibits phosphorylation of the receptor [9].

Chemical compound and disease context of CCRIS 3927

• Using the sucrose density gradient technique, we have compared androgen receptor values obtained in the presence and absence of sodium molybdate from cytosols of surgically obtained human prostate cancer tissue [2].
• Protective mechanism of sodium molybdate against the acute toxicity of cadmium in rats. II. Prevention of cytoplasmic acidification [10].
• Protective effect of sodium molybdate on the acute toxicity of mercuric chloride. V. Enhancement of renal regeneration after exposure to HgCl2 [11].
• The effect of lanthanum sulphate together with 100 micrometer of sodium molybdate show that there is considerable increase in nitrogen fixation and carbon consumption in the culture medium of nitrogen-fixing Azotobacter species A1 and A2, isolated from Allahabad soil [12].
• Most investigators comparing ligand-binding procedures for quantifying estrogen and progestin receptors in human breast cancer with procedures employing monoclonal antibody-based methods have utilized an inappropriate variety of reaction conditions, including the elimination of sodium molybdate in the steroid-binding assays [13].

Biological context of CCRIS 3927

• This high concentration of specific binding sites was present only if the placental tissue was initially homogenized in a buffer containing sodium molybdate (10 or 20 mM) [14].
• The binding protein in UISO-MEL-2 and -4 sedimented at 8.5S and 9.2S, respectively, in the presence of 10 mM sodium molybdate [15].
• Mercury methylation in Polygonum densiflorum and Eichhornia crassipes was reduced when SRB metabolism was inhibited by sodium molybdate [16].
• These preliminary data suggest that sodium molybdate induces dominant lethality at the postmeiotic stage of spermatogenesis [3].
• Effect of sodium molybdate on the status of lipids, lipid peroxidation and antioxidant systems in alloxan-induced diabetic rats [17].

Anatomical context of CCRIS 3927

• Fractionation of sodium molybdate-stabilized gallbladder cytosol on Sephadex G-100 demonstrates that the labeled hormones are not bound to defined proteins such as sex steroid binding globulin or albumin [18].
• Progesterone receptor from the chick oviduct was characterized in the presence of sodium molybdate, an agent which blocks receptor transformation and maintains the receptor in an aggregated state [19].
• We have investigated the stability of the [3H]dexamethasone 21-mesylate-labeled nonactivated glucocorticoid-receptor complex in rat thymus cytosol containing 20 mM sodium molybdate [20].
• Action of glycerol and sodium molybdate in stabilization of the progesterone receptor from rat trophoblast [21].
• The inclusion of sodium molybdate in the homogenization buffer led to an increased uptake of [3H]dexamethasone by amnion and choriodecidua; the specific binding ranged from 9-25 fmol/mg protein for cytosol and was 8 fmol/mg protein for nuclear preparations [22].

Associations of CCRIS 3927 with other chemical compounds

• Treatment of cytosols with 20 mM sodium molybdate or 50 mM leupeptin did not change the chromatofocusing profiles [23].
• In the presence of sodium molybdate, the macromolecule has a sedimentation coefficient of 8.3 S in sucrose gradients of low ionic strength, a Stokes radius of 56.6 A (Sephacryl S-300 columns), a molecular weight of approximately 192,000, a frictional ratio of 1.5, and an axial ratio of 8 [24].
• Inhibition of uterine estrogen receptor transformation by sodium molybdate [25].
• In vitro reconstitution of the purified apoprotein was achieved using an incubation mixture containing purified precursor Z, purified MPT synthase, and sodium molybdate [26].
• Thermal inactivation of the unoccupied progesterone receptor is inhibited by 10 mM sodium molybdate, whereas thermal inactivation of the ammonium sulfate-purified progesterone receptor is not [27].

Gene context of CCRIS 3927

• With a modified assay (the presence of sodium molybdate and a reduction in the amount of charcoal used to adsorb "excess" ligand) we found that cytosol from LS180 cells contains a high concentration of AhR (400-500 fmol/mg cytosolic protein) when detected by [3H]TCDD or [3H]MC [28].
• Routine addition of sodium molybdate to samples analyzed for PR by centrifugation overnight can be expected to yield an increased percent of receptor-positive patients with higher average concentration of receptor, compared with results of previous methodology [29].
• The hormone-dependent interaction of vaults with estrogen receptor was reproducible in vitro and was prevented by sodium molybdate [30].
• Expression of the human cDNA in a rabbit reticulocyte lysate system allowed functional analysis of ligand binding, agonist-induced and Ah receptor nuclear translocator-dependent DNA binding, and receptor stabilization by sodium molybdate [31].
• After the inclusion of sodium molybdate in the reconstitution mixture, active sulfite oxidase was obtained, revealing that in vitro MPT synthase and aposulfite oxidase are sufficient for the insertion of MPT into sulfite oxidase and the conversion of MPT into Moco in the presence of high concentrations of molybdate [26].

Analytical, diagnostic and therapeutic context of CCRIS 3927

• We have investigated the physiochemical characteristics of trypsin-treated, molybdate-stabilized glucocorticoid-receptor complexes from rat liver in the presence of 10 mM sodium molybdate by high performance ion-exchange chromatography, high performance size-exclusion chromatography, and sedimentation analysis [32].
• The cytosol receptor prepared in the presence of sodium molybdate remains in the non-transformed 8S state following affinity chromatography [33].
• Rapid centrifugation with a vertical tube rotor showed both 8-9 S and 4-5 S receptor species in the presence of 10 mM sodium molybdate with a 90-96% recovery [34].
• The activating effect of ATP on the glucocorticoid-receptor complexes and sensitivity of this activation to sodium molybdate was also confirmed by DEAE-cellulose chromatography of cytosolic receptor preparations [35].

References