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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Effect of oral administration of tri-o-cresyl phosphate on in vitro phosphorylation of membrane and cytosolic proteins from chicken brain.

The effects of a single oral dose of 750 mg/kg tri-o-cresyl phosphate (TOCP) on the endogenous phosphorylation of specific brain proteins were assessed in male adult chickens following the development of delayed neurotoxicity. Phosphorylation of crude synaptosomal (P2) membrane and synaptosomal cytosolic proteins was assayed in vitro by using [gamma-32P]ATP as phosphate donor. Following resolution of brain proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis, specific protein phosphorylation was detected by autoradiography and quantified by microdensitometry. TOCP administration enhanced the phosphorylation of both cytosolic (Mr 65,000 and 55,000) and membrane (20,000) proteins by as much as 146% and 200%, respectively.[1]


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