Identification of 3,4-dihydroxy-5-hexaprenylbenzoic acid as an intermediate in the biosynthesis of ubiquinone-6 by Saccharomyces cerevisiae.
The mutant strain of Saccharomyces cerevisiae E3-24 is unable to synthesize ubiquinone-6. When this mutant is grown in the presence of p-hydroxy[U-14C]benzoate or p-hydroxy[carboxy-14C]benzoate, a radioactive compound accumulates. This new metabolite has been isolated and identified as 3,4-dihydroxy-5-hexaprenylbenzoate (3,4-DHHB). Aerobically grown prototrophic strains of S. cerevisiae were found to contain only low levels of this compound. When strain X963-18C, blocked at homoserine O-transacetylase (in methionine biosynthesis), was deprived of methionine, ubiquinone biosynthesis ceased, and 3,4-DHHB was observed to accumulate. This suggested that S-adenosylmethionine (SAM) could be the methyl donor for 3,4-DHHB. Restoration of methionine to the cultures released this block and resulted in the conversion of 3,4-DHHB to ubiquinone-6, demonstrating a precursor--product relationship. The identification of 3,4-DHHB as an intermediate in ubiquinone biosynthesis in yeast establishes an alternate pathway for ubiquinone biosynthesis in eukaryotes.[1]References
- Identification of 3,4-dihydroxy-5-hexaprenylbenzoic acid as an intermediate in the biosynthesis of ubiquinone-6 by Saccharomyces cerevisiae. Goewert, R.R., Sippel, C.J., Olson, R.E. Biochemistry (1981) [Pubmed]
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